High impact information on KAR5

• KAR5 is required for membrane fusion during karyogamy, the process of nuclear fusion during yeast mating [1].
• Kar5p is induced as part of the pheromone response pathway, suggesting that this protein uniquely plays a specific role during mating in nuclear membrane fusion [1].
• Kar5p is a membrane protein with its soluble domain entirely contained within the lumen of the endoplasmic reticulum [1].
• In pheromone-treated cells, Kar5p was localized to the vicinity of the spindle pole body, the initial site of fusion between haploid nuclei during karyogamy [1].
• Moreover, in sec71 mutants, Kar5p was absent from the SPB and was not detected by Western blot or immunoprecipitation of pulse-labeled protein [2].

Other interactions of KAR5

• Analysis of double mutants suggested that Kar5p acts before Kar8/Jem1p [2].

References