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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Gene Review: 

NIP1  -  Nip1p

Saccharomyces cerevisiae S288c

Synonyms: Eukaryotic translation initiation factor 3 93 kDa subunit, Eukaryotic translation initiation factor 3 subunit C, Nuclear transport protein NIP1, Translation initiation factor eIF3, p93 subunit, YM9924.01C, ...
 
 
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High impact information on NIP1

  • The TIF32-CTD interacted in vitro with helices 16-18 of domain I in 18S rRNA, and the TIF32-NTD and NIP1 interacted with 40S protein RPS0A [1].
  • The yeast eIF3 subunits TIF32/a, NIP1/c, and eIF5 make critical connections with the 40S ribosome in vivo [1].
  • In addition to the contact between eIF2 and the N-terminal domain (NTD) of NIP1 bridged by eIF5, the C-terminal domain (CTD) of TIF32 binds eIF2 directly and is required for eIF2-eIF3 association in vivo [2].
  • Thus, several key functions of eIF3 can be carried out by the PRT1-TIF32-NIP1 subcomplex [3].
  • NIP1, a gene required for nuclear transport in yeast [4].
 

Biological context of NIP1

  • NIP1+ was shown to be an essential gene by gene disruption experiments [4].
  • Both effects of this NIP1 mutation were suppressed by eIF1 overexpression, as was the Sui(-) phenotype conferred by eIF5-G31R [5].
  • Analysis of the cloned cDNA encoding p110 indicates that its amino acid sequence is 31% identical to that of the yeast protein, Nip1 [6].
 

Associations of NIP1 with chemical compounds

  • Furthermore, an epitope-labeled NIP1 protein migrated in SDS/polyacrylamide gels with a mass of approximately 100,000 Da and was shown by immunofluorescence to localize mainly in the cytoplasm [4].
 

Physical interactions of NIP1

  • The N-terminal domain (NTD) of NIP1/eIF3c interacts directly with eIF1 and eIF5 and indirectly through eIF5 with the eIF2-GTP-Met-tRNA(i)(Met) ternary complex (TC) to form the multifactor complex (MFC) [5].
 

Other interactions of NIP1

  • His8-PRT1 and NIP1 did not form a stable binary subcomplex [3].

References

  1. The yeast eIF3 subunits TIF32/a, NIP1/c, and eIF5 make critical connections with the 40S ribosome in vivo. Valásek, L., Mathew, A.A., Shin, B.S., Nielsen, K.H., Szamecz, B., Hinnebusch, A.G. Genes Dev. (2003) [Pubmed]
  2. Direct eIF2-eIF3 contact in the multifactor complex is important for translation initiation in vivo. Valásek, L., Nielsen, K.H., Hinnebusch, A.G. EMBO J. (2002) [Pubmed]
  3. A subcomplex of three eIF3 subunits binds eIF1 and eIF5 and stimulates ribosome binding of mRNA and tRNA(i)Met. Phan, L., Schoenfeld, L.W., Valásek, L., Nielsen, K.H., Hinnebusch, A.G. EMBO J. (2001) [Pubmed]
  4. NIP1, a gene required for nuclear transport in yeast. Gu, Z., Moerschell, R.P., Sherman, F., Goldfarb, D.S. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  5. Interactions of eukaryotic translation initiation factor 3 (eIF3) subunit NIP1/c with eIF1 and eIF5 promote preinitiation complex assembly and regulate start codon selection. Valásek, L., Nielsen, K.H., Zhang, F., Fekete, C.A., Hinnebusch, A.G. Mol. Cell. Biol. (2004) [Pubmed]
  6. Conservation and diversity of eukaryotic translation initiation factor eIF3. Asano, K., Kinzy, T.G., Merrick, W.C., Hershey, J.W. J. Biol. Chem. (1997) [Pubmed]
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