High impact information on SPO1

• The sporulation-specific enzymes encoded by the DIT1 and DIT2 genes catalyze a two-step reaction leading to a soluble LL-dityrosine-containing precursor of the yeast spore wall [1].
• Dityrosine is a sporulation-specific component of the yeast ascospore wall that is essential for the resistance of the spores to adverse environmental conditions [1].
• Thus, in wild-type strains, the increased expression of IME1 in starved a/alpha cells can account entirely for cell type control, but only partially for nutritional control, of early sporulation-specific gene expression [2].
• A second sporulation-specific gene, designated SPO16, was found adjacent to SPO12 and shown to be necessary for efficient spore formation [3].
• We found that the introduction into a yeast cell of a high-copy-number plasmid containing the 5' end of the SPS2 gene, a sporulation-specific gene of Saccharomyces cerevisiae, led to a reduction in the efficiency of spore formation [4].

Biological context of SPO1

• The phenotype of a complete deletion indicates that SPO1 is dispensable for vegetative growth, premeiotic DNA synthesis and meiotic recombination [5].
• At present SPO1 is the only known gene required for SPB duplication in meiosis but not in mitosis [5].
• Alteration of a conserved serine residue in the putative phospholipase active site, and presence of the spo1-1 temperature-sensitive mutation, which resides near this site, each result in loss of SPO1 function [5].
• The SPO1 gene product required for meiosis in yeast has a high similarity to phospholipase B enzymes [6].
• ORF N2016 is immediately adjacent to the centromere, suggesting that it corresponds to the SPO1 gene, which is very tightly linked to the centromere at the left arm side of chromosome XIV (Mortimer et al., 1989) [7].

Anatomical context of SPO1

• Here we report in-depth analysis of the SPO1 gene including its transcriptional control by regulators of early gene expression, protein localization to the ER lumen and periplasmic space, and molecular genetic studies of its role in meiosis [8].

Associations of SPO1 with chemical compounds

• This protein was found to be identical in size and charge to a major, sporulation-specific protein identified in a two-dimensional polyacrylamide gel electrophoretic comparison of the in vitro translation products of total RNA from sporulating MATa/MAT alpha cells and asporogenous MAT alpha/MAT alpha cells [9].
• SPS19 shares with the sporulation-specific SPS18 a common promoter region that contains an oleate response element [10].

Other interactions of SPO1

• We previously identified a transcriptional regulatory element, which we call NRE(DIT), that is required for repression of the sporulation-specific genes, DIT1 and DIT2, during vegetative growth of Saccharomyces cerevisiae [11].
• A single gene, CWP1, was recovered as a multicopy suppressor of the spo1 null [5].
• Both mutants were blocked before premeiotic DNA synthesis, and both complemented spo1, spo3, and spo7 [12].
• The SPS4 gene of Saccharomyces cerevisiae encodes a major sporulation-specific mRNA [9].
• The Saccharomyces STA2 and SGA genes, encoding the extracellular and intracellular sporulation-specific glucoamylase respectively, have been cloned and their transcription and regulation studied [13].

Analytical, diagnostic and therapeutic context of SPO1

• A search for additional S. cerevisiae septin genes using the polymerase chain reaction identified SPR3, a gene that had been identified previously on the basis of its sporulation-specific expression [14].
• By two-dimensional thin-layer chromatography, eight compounds were resolved, one of which was sporulation specific [15].

References