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NOP4  -  Nop4p

Saccharomyces cerevisiae S288c

Synonyms: NOP77, Nucleolar protein 4, Nucleolar protein NOP77, YPL043W
 
 
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High impact information on NOP4

  • The yeast NOP4 gene product is an essential nucleolar protein required for pre-rRNA processing and accumulation of 60S ribosomal subunits [1].
  • To examine the function of Nop4p, we constructed a conditional null allele of NOP4 by placing this gene under the control of the glucose-repressible GAL1 promoter [1].
  • NOP4 encodes a 78 kDa protein that contains two prototypical RNA recognition motifs (RRMs) flanking an imperfect RRM lacking characteristic RNP1 and RNP2 motifs [1].
  • The Saccharomyces cerevisiae NOP4 gene was isolated by screening a lambda gt11 yeast genomic DNA library with a monoclonal antibody against a yeast nucleolar protein [1].
  • Nop4 protein depletion results in diminished accumulation of 60S ribosomal subunits, assignable to a defect in ribosome biogenesis arising from a lack of production of mature 25S rRNA from 27S precursor rRNA [1].
 

Biological context of NOP4

  • When cells are shifted from galactose-containing medium to glucose-containing medium, NOP4 transcription is terminated, Nop4 protein is depleted and cell growth is impaired [1].
  • NOP77p contains three canonical RNA recognition motifs (RRMs), suggesting that it is an RNA binding protein [2].
  • Depletion of NOP77p impairs both the processing and methylation of the pre-rRNA [2].
  • Our results indicate that each RRM is essential for Nop4p function; mutations in conserved aromatic residues of Nop4p cause a temperature-sensitive lethal phenotype and diminished 60 S ribosomal subunit production [3].
 

Associations of NOP4 with chemical compounds

  • The NOP77 allele which complements the synthetic lethal nop1 strains has an alanine at position 308, predicted to lie in helix alpha 1 of RRM3, whereas the non-complementing nop77-1 allele contains a proline at the corresponding position [2].

References

 
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