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Gene Review

DNF1  -  aminophospholipid-translocating P4-type...

Saccharomyces cerevisiae S288c

Synonyms: Flippase DNF1, Phospholipid-transporting ATPase DNF1, SYGP-ORF7, YER166W
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Disease relevance of DNF1

  • The weight gain of the HF (high fat diet) (162.58 +/- 6.68 g) was significantly (p < 0.05) greater than that of DNF-1, DNF-2, (high fat diet with DNF of 0.5 and 1.0 g/kg body weight, respectively) and control groups (143.19 +/- 7.33 g, 139.20 +/- 8.36 g, 130.23 +/- 8.02 g, respectively) [1].

High impact information on DNF1


Anatomical context of DNF1


Associations of DNF1 with chemical compounds

  • Thus, flip of phosphatidylcholine molecules with three different Bodipy fluorophores (Bodipy FL, Bodipy 530, and Bodipy 581) was tested and compared with that of NBD-PC in strains carrying deletions in LEM3, DNF1, and DNF2 [5].
  • It was also found that the triacylglycerol (TG) levels decreased significantly (p < 0.05) in the DNF-2 group from that of the HF, but there was no significant (p < 0.05) difference between DNF-1 and DNF-2 [1].

Other interactions of DNF1

  • Interestingly, these lem3 mutants exhibited nearly normal levels of NBD-labeled phospholipid internalization across the plasma membrane, suggesting that Lem3p may have other functions in addition to regulation of the putative APLT activity of Dnf1p at the plasma membrane [6].

Analytical, diagnostic and therapeutic context of DNF1

  • The wet weight of the epididymal fat and the perirenal fat pads of the DNF-1, DNF-2 and control groups were reduced significantly (p < 0.05) [1].


  1. Yeast hydrolysate reduces body fat of dietary obese rats. Kim, K.M., Chang, U.J., Kang, D.H., Kim, J.M., Choi, Y.M., Suh, H.J. Phytotherapy research : PTR. (2004) [Pubmed]
  2. Yeast P4-ATPases Drs2p and Dnf1p Are Essential Cargos of the NPFXD/Sla1p Endocytic Pathway. Liu, K., Hua, Z., Nepute, J.A., Graham, T.R. Mol. Biol. Cell (2007) [Pubmed]
  3. Loss of P4 ATPases Drs2p and Dnf3p disrupts aminophospholipid transport and asymmetry in yeast post-Golgi secretory vesicles. Alder-Baerens, N., Lisman, Q., Luong, L., Pomorski, T., Holthuis, J.C. Mol. Biol. Cell (2006) [Pubmed]
  4. Drs2p-related P-type ATPases Dnf1p and Dnf2p are required for phospholipid translocation across the yeast plasma membrane and serve a role in endocytosis. Pomorski, T., Lombardi, R., Riezman, H., Devaux, P.F., van Meer, G., Holthuis, J.C. Mol. Biol. Cell (2003) [Pubmed]
  5. Fluorescent, acyl chain-labeled phosphatidylcholine analogs reveal novel transport pathways across the plasma membrane of yeast. Elvington, S.M., Bu, F., Nichols, J.W. J. Biol. Chem. (2005) [Pubmed]
  6. Mutational analysis of the Lem3p-Dnf1p putative phospholipid-translocating P-type ATPase reveals novel regulatory roles for Lem3p and a carboxyl-terminal region of Dnf1p independent of the phospholipid-translocating activity of Dnf1p in yeast. Noji, T., Yamamoto, T., Saito, K., Fujimura-Kamada, K., Kondo, S., Tanaka, K. Biochem. Biophys. Res. Commun. (2006) [Pubmed]
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