Disease relevance of repB

• RepB was overproduced in an Escherichia coli expression system and the protein was purified and characterized [1].

High impact information on repB

• This site is included in a region that contains the promoter for the repA and repB genes and is contiguous to the plasmid ori(+) [2].
• Transcriptional and translational fusions, together with in vitro inhibition of RepB synthesis by specific oligonucleotides, showed translational inhibition of RepB synthesis by RNA II, perhaps by directly blocking the accessibility of the ribosomes to the repB initiation of translation signals [3].
• Binding of CopG to its operator results in repression, at the transcriptional level, of its own synthesis and that of the initiator of replication protein, RepB [4].
• However, as a striking difference with other Gram-positive replicons, the nick generated by RepB lies 86 base-pairs upstream from its binding region [1].
• Footprinting experiments showed that RepB protein binds to a DNA region that includes three direct repeats of 11 base-pairs [1].

Associations of repB with chemical compounds

• The streptococcal plasmid pMV158 has been reported to harbor five genes: three involved in initiation of rolling circle replication and its control (copG, repB, and maII), one involved in conjugative mobilization (mobM), and the fifth one specifying constitutive resistance to tetracycline (tet) [5].

Other interactions of repB

• ORF1 and ORF2 encode for proteins highly homologous to CopG and RepB of the pMV158 family, respectively [6].

References