The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

rocF  -  arginase

Helicobacter pylori J99

 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of rocF

  • The H. pylori gene hp1399, which is orthologous to the Bacillus subtilis rocF gene encoding arginase, was cloned, and isogenic allelic exchange mutants of three H. pylori strains were made by using two different constructs: 236-2 and rocF::aphA3 [1].
  • The gastric human pathogen, Helicobacter pylori, also has a complete urea cycle and contains the rocF gene encoding arginase (RocF), which is involved in the pathogenesis of H. pylori infection [2].
  • Antibodies raised to purified His(6)-RocF reacted with both H. pylori and E. coli extracts containing arginase, but not with extracts from rocF mutants of H. pylori or E. coli lacking the rocF gene [2].
 

High impact information on rocF

  • The substantially higher levels of NO generated by macrophages cocultured with rocF-deficient H. pylori resulted in efficient killing of the bacteria, whereas wild-type H. pylori exhibited no loss of survival under these conditions [3].
  • Coculture of live H. pylori with T cells demonstrated that the wild-type strain, but not the arginase mutant rocF(-), depleted L-arginine and caused a decrease in CD3zeta expression [4].
  • An H. pylori arginase attenuates host inflammation, a thioredoxin required as a reductant for many oxidative stress enzymes is also a chaperon, and some novel properties of KatA and AhpC were discovered [5].
  • In this study, by global transcriptional profiling of an ArsS-deficient H. pylori mutant grown at pH 5.0, we define the ArsR approximately P-dependent regulon consisting of 109 genes, including the urease gene cluster, the genes encoding the aliphatic amidases AmiE and AmiF, and the rocF gene encoding arginase [6].
  • Urease activity was similar in both strains and both survived acid exposure equally well when exogenous urea was added, indicating that rocF is not required for urease activity in vitro [1].
 

Biological context of rocF

 

Anatomical context of rocF

  • Some enzymes of eukaryotic origin are spread throughout the arginase part of the trees: they might have been derived from the genes found in the early symbiotic bacteria that became the organelles [8].
 

Associations of rocF with chemical compounds

  • Cell suspensions, lysates and membrane preparations generated L-ornithine and ammonium at high rates in incubations with L-arginine, indicating the presence of arginase activity [9].
 

Analytical, diagnostic and therapeutic context of rocF

References

  1. Helicobacter pylori rocF is required for arginase activity and acid protection in vitro but is not essential for colonization of mice or for urease activity. McGee, D.J., Radcliff, F.J., Mendz, G.L., Ferrero, R.L., Mobley, H.L. J. Bacteriol. (1999) [Pubmed]
  2. Purification and characterization of Helicobacter pylori arginase, RocF: unique features among the arginase superfamily. McGee, D.J., Zabaleta, J., Viator, R.J., Testerman, T.L., Ochoa, A.C., Mendz, G.L. Eur. J. Biochem. (2004) [Pubmed]
  3. Helicobacter pylori arginase inhibits nitric oxide production by eukaryotic cells: a strategy for bacterial survival. Gobert, A.P., McGee, D.J., Akhtar, M., Mendz, G.L., Newton, J.C., Cheng, Y., Mobley, H.L., Wilson, K.T. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  4. Helicobacter pylori arginase inhibits T cell proliferation and reduces the expression of the TCR zeta-chain (CD3zeta). Zabaleta, J., McGee, D.J., Zea, A.H., Hernández, C.P., Rodriguez, P.C., Sierra, R.A., Correa, P., Ochoa, A.C. J. Immunol. (2004) [Pubmed]
  5. The diverse antioxidant systems of Helicobacter pylori. Wang, G., Alamuri, P., Maier, R.J. Mol. Microbiol. (2006) [Pubmed]
  6. Characterization of the ArsRS Regulon of Helicobacter pylori, Involved in Acid Adaptation. Pflock, M., Finsterer, N., Joseph, B., Mollenkopf, H., Meyer, T.F., Beier, D. J. Bacteriol. (2006) [Pubmed]
  7. In situ characterization of Helicobacter pylori arginase. Mendz, G.L., Holmes, E.M., Ferrero, R.L. Biochim. Biophys. Acta (1998) [Pubmed]
  8. Phylogeny of related functions: the case of polyamine biosynthetic enzymes. Sekowska, A., Danchin, A., Risler, J.L. Microbiology (Reading, Engl.) (2000) [Pubmed]
  9. The urea cycle of Helicobacter pylori. Mendz, G.L., Hazell, S.L. Microbiology (Reading, Engl.) (1996) [Pubmed]
 
WikiGenes - Universities