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Gene Review:

ECs3549 - S-ribosylhomocysteinase

Escherichia coli O157:H7 str. Sakai

Zhu, J. et al., Guianvarc'h, D. et al., Wang, L. et al., Day, W.A. et al., Azakami, H. et al.

Azakami, Teramura, Matsunaga, Akimichi, Noiri, Ebisu, Kato,

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Disease relevance of ECs3549

However, AI-2 does not influence invasion operon expression and is not required for Shigella virulence, as mutants deficient in AI-2 synthesis are fully virulent [1].
In this report, we show that both AI-2 synthesis and uptake in Escherichia coli are subject to catabolite repression through the cyclic AMP (cAMP)-CRP complex, which directly stimulates transcription of the lsr (for "luxS regulated") operon and indirectly represses luxS expression [2].
An ortholog of luxS, the gene required for AI-2 synthesis in Vibrio harveyi, was isolated from the E. corrodens genome [3].

High impact information on ECs3549

Catalytic mechanism of s-ribosylhomocysteinase: ionization state of active-site residues [4].
S-Ribosylhomocysteinase (LuxS) catalyzes the cleavage of the thioether linkage in S-ribosylhomocysteine (SRH) to produce homocysteine (Hcys) and 4,5-dihydroxy-2,3-pentanedione (DPD), the precursor of type II bacterial autoinducer (AI-2) [4].
Here, we report the adaptation to the E. coli CFAS of a previously reported enzyme-coupled colorimetric assay based on the quantification, using Ellman's reagent, of homocysteine produced from S-adenosyl-L-homocysteine, a product of the reaction, in the presence of AdoHcy nucleosidase and S-ribosylhomocysteinase [5].

References

Shigella flexneri LuxS quorum-sensing system modulates virB expression but is not essential for virulence. Day, W.A., Maurelli, A.T. Infect. Immun. (2001) [Pubmed]
Cyclic AMP (cAMP) and cAMP receptor protein influence both synthesis and uptake of extracellular autoinducer 2 in Escherichia coli. Wang, L., Hashimoto, Y., Tsao, C.Y., Valdes, J.J., Bentley, W.E. J. Bacteriol. (2005) [Pubmed]
Characterization of autoinducer 2 signal in Eikenella corrodens and its role in biofilm formation. Azakami, H., Teramura, I., Matsunaga, T., Akimichi, H., Noiri, Y., Ebisu, S., Kato, A. J. Biosci. Bioeng. (2006) [Pubmed]
Catalytic mechanism of s-ribosylhomocysteinase: ionization state of active-site residues. Zhu, J., Knottenbelt, S., Kirk, M.L., Pei, D. Biochemistry (2006) [Pubmed]
Identification of new inhibitors of E. coli cyclopropane fatty acid synthase using a colorimetric assay. Guianvarc'h, D., Drujon, T., Leang, T.E., Courtois, F., Ploux, O. Biochim. Biophys. Acta (2006) [Pubmed]

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