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Gene Review

nusA  -  transcription elongation factor NusA

Escherichia coli O157:H7 str. Sakai

 
 
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Disease relevance of ECs4050

 

High impact information on ECs4050

  • By using this dipeptide system, we studied the effects of RNA polymerase holoenzyme and L factor (nus A gene product) on fMET-Val synthesis and compared the relative effects of the primary and secondary promoters in the L10 operon on expression of the beta-subunit gene [3].
  • When composite DNA constructed from L factor and a foreign gene was introduced into mouse embryonal carcinoma (F9) cells by transfection, the DNA was reestablished in the cells as a plasmid [2].
  • In other families, when the affected member had less than 1 nmol/L factor IX antigen, heterozygosity for Thr/Ala alleles excluded the carrier state even when DNA studies were not informative [4].
  • L-factor also extended the pause half-life of the pause obtained with the ilvGEDA template; however, addition of guanosine 5'-diphosphate,3'-diphosphate did not [5].
  • The E. coli L-factor enhances transcription pausing at this site (Farnham, P. J., Greenblatt, J., and Platt, T. (1982) Cell 29, 945-951); presumably it does so by facilitating recognition of the RNA hairpin by polymerase [6].
 

Biological context of ECs4050

References

  1. L factor that is required for beta-galactosidase synthesis is the nusA gene product involved in transcription termination. Greenblatt, J., Li, J., Adhya, S., Friedman, D.I., Baron, L.S., Redfield, B., Kung, H.F., Weissbach, H. Proc. Natl. Acad. Sci. U.S.A. (1980) [Pubmed]
  2. Establishment of composite DNA derived from L factor as a plasmid in mouse embryonal carcinoma (F9) cells. Nishimori, K., Kohda, T., Fujiwara, J., Oishi, M. Mol. Cell. Biol. (1988) [Pubmed]
  3. In vitro synthesis of the first dipeptide of the beta subunit of Escherichia coli RNA polymerase. Peacock, S., Cenatiempo, Y., Robakis, N., Brot, N., Weissbach, H. Proc. Natl. Acad. Sci. U.S.A. (1982) [Pubmed]
  4. Carrier testing in hemophilia B with an immunoassay that distinguishes a prevalent factor IX dimorphism. Smith, K.J., Thompson, A.R., McMullen, B.A., Frazier, D., Lin, S.W., Stafford, D., Kisiel, W., Thibodeau, S.N., Chen, S.H., Smith, L.F. Blood (1987) [Pubmed]
  5. Pausing of RNA polymerase during in vitro transcription through the ilvB and ilvGEDA attenuator regions of Escherichia coli K12. Hauser, C.A., Sharp, J.A., Hatfield, L.K., Hatfield, G.W. J. Biol. Chem. (1985) [Pubmed]
  6. A complementary DNA oligomer releases a transcription pause complex. Fisher, R., Yanofsky, C. J. Biol. Chem. (1983) [Pubmed]
  7. Prokaryotic gene expression in vitro: transcription-translation coupled systems. Cenatiempo, Y. Biochimie (1986) [Pubmed]
 
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