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Gene Review

ompA  -  major outer membrane protein

Chlamydophila pneumoniae J138

 
 
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Disease relevance of ompA

 

High impact information on ompA

  • As PorB was found to be in the outer membrane, as well as having weak structural characteristics similar to major outer membrane protein (MOMP) and other porins, a liposome-swelling assay was used to determine whether this protein had pore-forming capabilities [6].
  • A predicted protein (CT713) with weak sequence similarity to the major outer membrane protein (20.4% identity) in Chlamydia trachomatis was identified by Chlamydia genome analysis [6].
  • Nucleotide sequence analysis of the C. pneumoniae MOMP gene (ompA) revealed that it consisted of a 1,167-base open reading frame with an inferred 39,344-dalton mature protein of 366 amino acids plus a 23-amino-acid leader sequence [7].
  • IHC detected the C. pneumoniae major outer membrane protein antigen in 73.8% of the specimens from the group treated with clarithromycin and 77.0% of the specimens from the group treated with placebo (P was not significant) [8].
  • All five assays could detect even lower target levels from spiked sputum, with the 16S rRNA assays performing better than the ompA-based nested PCR (10(-6) inclusion-forming units [IFU] were detected in four of four and two of four replicates by the 16S rRNA TETR PCR and the 16S rRNA nested PCR, respectively) [1].
 

Chemical compound and disease context of ompA

 

Biological context of ompA

  • The assays included two conventional PCRs, one targeting a cloned PstI fragment and one targeting the 16S rRNA gene; two nested PCRs, one targeting the 16S rRNA gene and one targeting ompA; and a touchdown enzyme time release (TETR) PCR, targeting the 16S rRNA gene [10].
  • This lack of allelic polymorphisms at ompAVD4 has now been observed for koala C. pneumoniae, human C. pneumoniae, guinea pig inclusion conjuctivitis C. psittaci and feline conjuctivitis C. psittaci and may be correlated to a lack of antibody response to the chlamydial major outer membrane protein (MOMP) in these same strain/host combinations [11].
  • Nucleotide sequence and taxonomic value of the major outer membrane protein gene of Chlamydia pneumoniae IOL-207 [12].
 

Associations of ompA with chemical compounds

 

Other interactions of ompA

 

Analytical, diagnostic and therapeutic context of ompA

  • We analyzed the peripheral blood mononuclear cell (PBMC) fractions from 60 healthy human blood donors for the presence of C. pneumoniae DNA (by ompA PCR) and chlamydial antigens (by genus- and species-specific monoclonal antibody staining) [15].
  • C. pneumoniae NASBA was capable of detecting between 100 and 1000 ompA RNA molecules and could detect 0.2 IFU of C. pneumoniae using the aequorin bioluminescent assay [16].
  • METHODS: Carotid endarterectomy specimens and preoperative peripheral blood mononuclear cells (PBMC) of 75 individuals with severe cerebrovascular atherosclerosis were analyzed by means of a C. pneumoniae-specific quantitative ompA-based real-time PCR TaqMan system [17].
  • To detect ompA, a nested-PCR assay was designed, whereas for omp9, a PCR-Enyzme immunoassay (PCR-EIA) depending on streptavidin-biotin capture and dig detection of the PCR products was performed [18].
  • Sequence analysis of the major outer membrane protein gene of Chlamydia pneumoniae [7].

References

  1. Comparison of a new quantitative ompA-based real-Time PCR TaqMan assay for detection of Chlamydia pneumoniae DNA in respiratory specimens with four conventional PCR assays. Apfalter, P., Barousch, W., Nehr, M., Makristathis, A., Willinger, B., Rotter, M., Hirschl, A.M. J. Clin. Microbiol. (2003) [Pubmed]
  2. Failure to detect Chlamydia pneumoniae DNA in cerebral aneurysmal sac tissue with two different polymerase chain reaction methods. Cagli, S., Oktar, N., Dalbasti, T., Erensoy, S., Ozdamar, N., Göksel, S., Sayiner, A., Bilgiç, A. J. Neurol. Neurosurg. Psychiatr. (2003) [Pubmed]
  3. Isolation and antimicrobial susceptibilities of chlamydial isolates from Western barred bandicoots. Kumar, S., Kutlin, A., Roblin, P., Kohlhoff, S., Bodetti, T., Timms, P., Hammerschlag, M.R. J. Clin. Microbiol. (2007) [Pubmed]
  4. Genetic characterization of a Chlamydophila pneumoniae isolate from an African frog and comparison to currently accepted biovars. Hotzel, H., Grossmann, E., Mutschmann, F., Sachse, K. Syst. Appl. Microbiol. (2001) [Pubmed]
  5. Design of a multiplex PCR for Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae and Chlamydophila pneumoniae to be used on sputum samples. Strålin, K., Bäckman, A., Holmberg, H., Fredlund, H., Olcén, P. APMIS (2005) [Pubmed]
  6. Characterization and functional analysis of PorB, a Chlamydia porin and neutralizing target. Kubo, A., Stephens, R.S. Mol. Microbiol. (2000) [Pubmed]
  7. Sequence analysis of the major outer membrane protein gene of Chlamydia pneumoniae. Perez Melgosa, M., Kuo, C.C., Campbell, L.A. Infect. Immun. (1991) [Pubmed]
  8. Effect of clarithromycin treatment on Chlamydia pneumoniae in vascular tissue of patients with coronary artery disease: a randomized, double-blind, placebo-controlled trial. Berg, H.F., Maraha, B., van der Zee, A., Gielis, S.K., Roholl, P.J., Scheffer, G.J., Peeters, M.F., Kluytmans, J.A. J. Clin. Microbiol. (2005) [Pubmed]
  9. Chlamydia pneumoniae in asymptomatic carotid atherosclerosis. Sessa, R., Di Pietro, M., Schiavoni, G., Galdiero, M., Cipriani, P., Romano, S., Zagaglia, C., Santino, I., Faccilongo, S., Del Piano, M. International journal of immunopathology and pharmacology. (2006) [Pubmed]
  10. Analytical sensitivity, reproducibility of results, and clinical performance of five PCR assays for detecting Chlamydia pneumoniae DNA in peripheral blood mononuclear cells. Mahony, J.B., Chong, S., Coombes, B.K., Smieja, M., Petrich, A. J. Clin. Microbiol. (2000) [Pubmed]
  11. Characterization of the koala biovar of Chlamydia pneumoniae at four gene loci--ompAVD4, ompB, 16S rRNA, groESL spacer region. Wardrop, S., Fowler, A., O'Callaghan, P., Giffard, P., Timms, P. Syst. Appl. Microbiol. (1999) [Pubmed]
  12. Nucleotide sequence and taxonomic value of the major outer membrane protein gene of Chlamydia pneumoniae IOL-207. Carter, M.W., al-Mahdawi, S.A., Giles, I.G., Treharne, J.D., Ward, M.E., Clark, I.N. J. Gen. Microbiol. (1991) [Pubmed]
  13. Chlamydia pneumoniae in PBMC: reproducibility of the OMPA nested touchdown PCR. Sessa, R., Schiavoni, G., Di Pietro, M., Petrucca, A., Cipriani, P., Puopolo, M., Zagaglia, C., Fallucca, S., Del Piano, M. International journal of immunopathology and pharmacology. (2005) [Pubmed]
  14. Chlamydia pneumoniae in a free-ranging giant barred frog (Mixophyes iteratus) from Australia. Berger, L., Volp, K., Mathews, S., Speare, R., Timms, P. J. Clin. Microbiol. (1999) [Pubmed]
  15. Detection of Chlamydia pneumoniae DNA and antigen in the circulating mononuclear cell fractions of humans and koalas. Bodetti, T.J., Timms, P. Infect. Immun. (2000) [Pubmed]
  16. Nucleic acid sequence based amplification (NASBA) of Chlamydia pneumoniae major outer membrane protein (ompA) mRNA with bioluminescent detection. Coombes, B.K., Mahony, J.B. Comb. Chem. High Throughput Screen. (2000) [Pubmed]
  17. No evidence of involvement of Chlamydia pneumoniae in severe cerebrovascular atherosclerosis by means of quantitative real-time polymerase chain reaction. Apfalter, P., Barousch, W., Nehr, M., Willinger, B., Rotter, M., Hirschl, A.M. Stroke (2004) [Pubmed]
  18. The investigation of Chlamydophila pneumoniae in patients with multiple sclerosis. Budak, F., Keçeli, S., Efendi, H., Budak, F., Vahaboğlu, H. Int. J. Neurosci. (2007) [Pubmed]
 
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