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Gene Review

sucB  -  dihydrolipoyltranssuccinase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0715, JW0716
 
 
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Disease relevance of sucB

 

High impact information on sucB

  • Exogenous lysine is the regulatory signal for lys gene expression and specifically serves as a ligand for LysM by altering its DNA binding affinity [5].
  • ATP-driven proton pumping activity is lost with the substitution of lys, ile, val, or glu for arginine 210 [6].
  • [Mutagenesis of a dibasic peptide (lys-lys) at codons 498-499, the site at which a tau'-'LacZ fusion protein was cleaved in vitro (1) had no effect on gamma formation in vivo, suggesting that cleavage observed in vitro is not the mechanism of gamma formation in vivo [7].
  • The putative lys tRNA (anticodon UUU) differs from rabbit liver lys tRNA at five positions [8].
  • This new gene of Mu has been named 'lig'. A 5 kb fragment responsible for the reported effects and localized between genes gam and lys of Mu genome has been cloned in pBR322 [9].
 

Chemical compound and disease context of sucB

  • The genes encoding both subunits of the succinyl-CoA synthetase of Escherichia coli have been identified as distal genes of the suc operon, which also encodes the dehydrogenase (Elo; sucA) and succinyltransferase (E2o; sucB) components of the 2-oxoglutarate dehydrogenase complex [10].
  • Hybrid plasmids which complement E. coli mutants for arg, his, lys, met, pdx, pyr and thr markers were identified from the gene bank [11].
 

Biological context of sucB

  • The absence of a typical terminator sequence and the presence of an IS-like sequence downstream of sucB suggest that there may be further gene(s) in the suc operon [1].
  • The sucB structural gene comprises 1209 base pairs (403 codons excluding the initiating AUG), and it is preceded by a 14-base-pair intercistronic region containing a good ribosomal binding site [1].
  • The Coxiella burnetii sucB gene encoding the dihydrolipoamide succinyltransferase (E2o) enzyme was cloned by immunological screening of a lambda EMBL3 genomic library prepared from strain Nine Mile DNA and sequenced [4].
  • Promoter-containing DNA fragments extending approximately 200 bp upstream and downstream of the 5' starts of the lys, I and P transcripts were cloned into a multicopy lacZ-expression plasmid [12].
  • The gene order is col-imm-lys confirming previous genetic data [13].
 

Analytical, diagnostic and therapeutic context of sucB

  • These results show that MS-lys is an excellent candidate for crystallization, folding and denaturation studies [14].
  • Recombinant MS-lys presented a globular structure, with an alpha-helical content of 57% as assessed by circular dichroism spectroscopy [14].

References

  1. Nucleotide sequence of the sucB gene encoding the dihydrolipoamide succinyltransferase of Escherichia coli K12 and homology with the corresponding acetyltransferase. Spencer, M.E., Darlison, M.G., Stephens, P.E., Duckenfield, I.K., Guest, J.R. Eur. J. Biochem. (1984) [Pubmed]
  2. Cloning, nucleotide sequence, and expression of the Brucella melitensis sucB gene coding for an immunogenic dihydrolipoamide succinyltransferase homologous protein. Zygmunt, M.S., Díaz, M.A., Teixeira-Gomes, A.P., Cloeckaert, A. Infect. Immun. (2001) [Pubmed]
  3. Cloning, sequencing, and oxygen regulation of the Rhodobacter capsulatus alpha-ketoglutarate dehydrogenase operon. Dastoor, F.P., Forrest, M.E., Beatty, J.T. J. Bacteriol. (1997) [Pubmed]
  4. Characterization of the Coxiella burnetti sucB gene encoding an immunogenic dihydrolipoamide succinyltransferase. Nguyen, S.V., To, H., Yamaguchi, T., Fukushi, H., Hirai, K. Microbiol. Immunol. (1999) [Pubmed]
  5. The Sulfolobus solfataricus Lrp-like protein LysM regulates lysine biosynthesis in response to lysine availability. Brinkman, A.B., Bell, S.D., Lebbink, R.J., de Vos, W.M., van der Oost, J. J. Biol. Chem. (2002) [Pubmed]
  6. Proton translocation by the F1F0ATPase of Escherichia coli. Mutagenic analysis of the a subunit. Cain, B.D., Simoni, R.D. J. Biol. Chem. (1989) [Pubmed]
  7. Programmed ribosomal frameshifting generates the Escherichia coli DNA polymerase III gamma subunit from within the tau subunit reading frame. Blinkowa, A.L., Walker, J.R. Nucleic Acids Res. (1990) [Pubmed]
  8. Using iodinated single-stranded M13 probes to facilitate rapid DNA sequence analysis--nucleotide sequence of a mouse lysine tRNA gene. Han, J.H., Harding, J.D. Nucleic Acids Res. (1983) [Pubmed]
  9. Restoration of ligase activity in E. coli K12 lig ts7 strain by bacteriophage Mu and cloning of a DNA fragment harbouring the Mu 'lig' gene. Ghelardini, P., Paolozzi, L., Liebart, J.C. Nucleic Acids Res. (1980) [Pubmed]
  10. Cloning and expression of the succinyl-CoA synthetase genes of Escherichia coli K12. Buck, D., Spencer, M.E., Guest, J.R. J. Gen. Microbiol. (1986) [Pubmed]
  11. Construction of a novel gene bank of Bacillus subtilis using a low copy number vector in Escherichia coli. Hasnain, S., Thomas, C.M. J. Gen. Microbiol. (1986) [Pubmed]
  12. Mutational analysis of a C-dependent late promoter of bacteriophage Mu. Chiang, L.W., Howe, M.M. Genetics (1993) [Pubmed]
  13. Nucleotide sequences from the colicin E8 operon: homology with plasmid ColE2-P9. Uchimura, T., Lau, P.C. Mol. Gen. Genet. (1987) [Pubmed]
  14. Biophysical characterization of an insect lysozyme from Manduca sexta. López-Zavala, A.A., de-la-Re-Vega, E., Calderón-Arredondo, S.A., García-Orozco, K.D., Velázquez, E.F., Islas-Osuna, M.A., Valdez, M.A., Sotelo-Mundo, R.R. Protein Pept. Lett. (2004) [Pubmed]
 
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