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Gene Review

recE  -  Rac prophage; exonuclease VIII, 5' to 3'...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1347, JW1344, rmuB, sbcA
 
 
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Disease relevance of recE

 

High impact information on recE

  • Mutations within this region of Gifsy-1 yield the classical 'Sbc' phenotype: they suppress the recombination defect of recB mutants, apparently by activating a normally silent recE-like gene [3].
  • Homologous pairing proteins encoded by the Escherichia coli recE and recT genes [4].
  • Both the frequency of transformation and the production of Tcr recombinants were decreased by recE mutations, while recA and recF mutations were slightly stimulatory (twofold) [5].
  • The N-terminal 294 codons of recE have been deleted and the remaining C-terminal codons have been fused to the N-terminal portion of another reading frame we call sfcA [6].
  • Restriction nuclease analysis of a second deletion, sbcA81, by Southern blot technique indicates that about 105 kb of DNA have been deleted and a transcriptional gene fusion has occurred between recE and the regulatory region of an E. coli chromosomal gene [6].
 

Chemical compound and disease context of recE

  • By co-infection of these phage into RecE+ EcoRI+ E.coli, we were able to introduce double-strand breaks into these vectors, allowing efficient homologous recombination (in up to 10% of bacteria) by the recE pathway and selection of the recombinants by resistance to ampicillin [7].
 

Biological context of recE

 

Other interactions of recE

  • A Tn10 insertion mutation previously thought to be in recE is located in recT and is renamed recT101::Tn10 [1].
  • Three of the deletions remove the entire N-terminal portion of recE, fusing the C-terminal portion to N-terminal portions of racC in frame [8].
  • Intraplasmidic recombination, but not interplasmidic recombination via the recE pathway is independent of a functional recA product [12].
  • In one case, inactivation of the sbcC gene leads to an increase in rearrangements, and in another, insertions near the recE gene lead to an even larger increase [13].
 

Analytical, diagnostic and therapeutic context of recE

References

  1. Genetic and molecular analyses of the C-terminal region of the recE gene from the Rac prophage of Escherichia coli K-12 reveal the recT gene. Clark, A.J., Sharma, V., Brenowitz, S., Chu, C.C., Sandler, S., Satin, L., Templin, A., Berger, I., Cohen, A. J. Bacteriol. (1993) [Pubmed]
  2. Characterization of the recombination function of coliphage phi 80 as analogous to the recE recombinative pathway of Escherichia coli. Reyes, O. Virology (1982) [Pubmed]
  3. Unsuspected prophage-like elements in Salmonella typhimurium. Figueroa-Bossi, N., Coissac, E., Netter, P., Bossi, L. Mol. Microbiol. (1997) [Pubmed]
  4. Homologous pairing proteins encoded by the Escherichia coli recE and recT genes. Kolodner, R., Hall, S.D., Luisi-DeLuca, C. Mol. Microbiol. (1994) [Pubmed]
  5. Intramolecular recombination of linear DNA catalyzed by the Escherichia coli RecE recombination system. Symington, L.S., Morrison, P., Kolodner, R. J. Mol. Biol. (1985) [Pubmed]
  6. Physical analysis of spontaneous and mutagen-induced mutants of Escherichia coli K-12 expressing DNA exonuclease VIII activity. Mahajan, S.K., Chu, C.C., Willis, D.K., Templin, A., Clark, A.J. Genetics (1990) [Pubmed]
  7. Generating molecular diversity by homologous recombination in Escherichia coli. Wang, P.L., Lo, B.K., Winter, G. Protein Eng. Des. Sel. (2005) [Pubmed]
  8. Suppression of a frameshift mutation in the recE gene of Escherichia coli K-12 occurs by gene fusion. Chu, C.C., Templin, A., Clark, A.J. J. Bacteriol. (1989) [Pubmed]
  9. Analysis of the recE locus of Escherichia coli K-12 by use of polyclonal antibodies to exonuclease VIII. Luisi-DeLuca, C., Clark, A.J., Kolodner, R.D. J. Bacteriol. (1988) [Pubmed]
  10. Isolation of an autonomously replicating DNA fragment from the region of defective bacteriophage PBSX of Bacillus subtilis. Anderson, L.M., Ruley, H.E., Bott, K.F. J. Bacteriol. (1982) [Pubmed]
  11. Equine herpesvirus type 1 devoid of gM and gp2 is severely impaired in virus egress but not direct cell-to-cell spread. Rudolph, J., Osterrieder, N. Virology (2002) [Pubmed]
  12. Interplasmidic and intraplasmidic recombination in Escherichia coli K-12. Laban, A., Cohen, A. Mol. Gen. Genet. (1981) [Pubmed]
  13. Genes involved in the determination of the rate of inversions at short inverted repeats. Slupska, M.M., Chiang, J.H., Luther, W.M., Stewart, J.L., Amii, L., Conrad, A., Miller, J.H. Genes Cells (2000) [Pubmed]
  14. Insertion of modifications in the beta-globin locus using GET recombination with single-stranded oligonucleotides and denatured PCR fragments. Jamsai, D., Orford, M., Fucharoen, S., Williamson, R., Ioannou, P.A. Mol. Biotechnol. (2003) [Pubmed]
 
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