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Gene Review

topB  -  DNA topoisomerase III

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1761, JW1752, mutR
 
 
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Disease relevance of topB

 

High impact information on topB

 

Biological context of topB

  • The products of the orf183 and topB genes were required neither for selenoprotein biosynthesis nor for selenation of tRNAs. selAB transcription was driven by a single, weak promoter; however, two major selD operon transcripts were identified [7].
  • Frameshift mutations in topB strain also significantly increased compared with those of wild-type (17 vs. 2%) [8].
  • The present data suggest that topB is a novel class of mutator that strongly induces repeated sequence dependent deletion mutagenesis and high frequencies of frameshift mutagenesis [8].
  • A 17-amino-acid residue domain has been identified in Escherichia coli DNA topoisomerase III (Topo III) that is essential for Topo III-mediated resolution of DNA replication intermediates in vitro [9].
 

Associations of topB with chemical compounds

  • (ii) Mutations in mutR can be induced with the frameshift mutagen ICR-191 [10].
 

Other interactions of topB

References

  1. Molecular cloning and DNA sequence analysis of Escherichia coli topB, the gene encoding topoisomerase III. DiGate, R.J., Marians, K.J. J. Biol. Chem. (1989) [Pubmed]
  2. Crystal structure of a complex of a type IA DNA topoisomerase with a single-stranded DNA molecule. Changela, A., DiGate, R.J., Mondragón, A. Nature (2001) [Pubmed]
  3. Type I topoisomerase activity is required for proper chromosomal segregation in Escherichia coli. Zhu, Q., Pongpech, P., DiGate, R.J. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  4. The traE gene of plasmid RP4 encodes a homologue of Escherichia coli DNA topoisomerase III. Li, Z., Hiasa, H., Kumar, U., DiGate, R.J. J. Biol. Chem. (1997) [Pubmed]
  5. Escherichia coli DNA topoisomerase III is a site-specific DNA binding protein that binds asymmetrically to its cleavage site. Zhang, H.L., Malpure, S., DiGate, R.J. J. Biol. Chem. (1995) [Pubmed]
  6. Characterization of Exc, a novel protein required for the excision of Bacteroides conjugative transposon. Sutanto, Y., Shoemaker, N.B., Gardner, J.F., Salyers, A.A. Mol. Microbiol. (2002) [Pubmed]
  7. Expression and operon structure of the sel genes of Escherichia coli and identification of a third selenium-containing formate dehydrogenase isoenzyme. Sawers, G., Heider, J., Zehelein, E., Böck, A. J. Bacteriol. (1991) [Pubmed]
  8. An Escherichia coli topB mutant increases deletion and frameshift mutations in the supF target gene. Uematsu, N., Eda, S., Yamamoto, K. Mutat. Res. (1997) [Pubmed]
  9. Identification of a unique domain essential for Escherichia coli DNA topoisomerase III-catalysed decatenation of replication intermediates. Li, Z., Mondragón, A., Hiasa, H., Marians, K.J., DiGate, R.J. Mol. Microbiol. (2000) [Pubmed]
  10. Further characterization of a non-essential mutator gene in Escherichia coli K-12. Hoess, R.H., Fan, D.P. J. Bacteriol. (1975) [Pubmed]
  11. Isolation of the topB gene encoding DNA topoisomerase III as a multicopy suppressor of topA null mutations in Escherichia coli. Broccoli, S., Phoenix, P., Drolet, M. Mol. Microbiol. (2000) [Pubmed]
 
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