Disease relevance of trmD

• The nucleotide sequence of a 4.6-kb SalI-EcoRI DNA fragment including the trmD operon, located at min 56 on the Escherichia coli K-12 chromosome, has been determined [1].
• The trmD and rplS genes from the Serratia marcescens trmD operon are presented [2].

High impact information on trmD

• The probable locations of promoter and terminator of the trmD operon are suggested [1].
• The translational start of the trmD gene was deduced from the known NH2-terminal amino acid sequence of the purified enzyme [1].
• The negative control element of 21K does not seem to be responsible for the low expression of the trmD gene located downstream [3].
• The trmD operon is a four-cistron operon in which the first and fourth genes encode ribosomal proteins S16 (rpsP) and L19 (rplS), respectively [4].
• The second gene encodes a 21,000 Mr polypeptide of unknown function and the third gene (trmD) encodes the enzyme tRNA(m1G37)methyltransferase, which catalyzes the formation of 1-methylguanosine (m1G) next to the 3' end of the anticodon (position 37) of some tRNAs in Escherichia coli [4].

Biological context of trmD

• The different parts of the trmD operon mRNA decay at a uniform rate [4].
• Possibly, the two mRNA secondary structures function to decouple translation of 21K and trmD from that of the respective upstream cistron in order to achieve their independent regulation [3].

Other interactions of trmD

• Functional analysis of the ffh-trmD region of the Escherichia coli chromosome by using reverse genetics [5].
• Here we have constructed translational gene fusions of the trmD operon and lacZ [6].

References