Disease relevance of hypE

• This gene, termed hydF, mapped at 59 min in the E. coli chromosome and resided next to the hydB gene [1].

High impact information on hypE

• This is encoded by three genes, hydC (gamma), hydB (beta) and hydA (alpha), organized within a single operon that contains five additional open reading frames (ORFs) [2].
• In mutants deficient in hydrogenase (hydB), ethanol production from sorbitol was greatly decreased, implying that hydrogen gas released from formate by the formate-hydrogen lyase system may be partially recycled, in the wild type, to increase the yield of the highly reduced fermentation product ethanol [3].
• The fhlA gene resides next to the hydB gene at 59 min in the E. coli chromosome, and the two genes are transcribed in opposite directions [4].
• Oxygen and nitrate repressed transcription of this gene. hydB gene transcription also required sigma 60, the product of the rpoN gene [5].
• One of the essential genes, hydB, is encoded by a DNA fragment of approximately 1.0 kilobase [5].

Biological context of hypE

• Plasmids pAK23 and pAK23S restored activity in another hydrogenase-negative strain, SE-3-1 (hydB), while plasmid pAK23C did not, suggesting that plasmid pAK23 contains two genes required for hydrogenase expression [6].
• The hydA and hydB genes, which map near minute 59 of the genome map, 17 kb distant from each other, are not structural hydrogenase genes, but mutation in either of these genes leads to failure to synthesize any of the hydrogenase isoenzymes [7].

Associations of hypE with chemical compounds

• It was found to be dependent on the positive control exerted by the fdhA, B and C genes, possibly involved in selenium incorporation, and by an hydB gene affecting the formate hydrogenlyase pathway [8].

References