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Gene Review

cysJ  -  sulfite reductase, alpha subunit,...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK2759, JW2734
 
 
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Disease relevance of cysJ

  • Mutagenesis and regulation of the cysJ promoter of Escherichia coli K-12 [1].
  • NADPH-sulfite reductase flavoprotein (SiR-FP) was purified from a Salmonella typhimurium cysG strain that does not synthesize the hemoprotein component of the sulfite reductase holoenzyme. cysJ, which codes for SiR-FP, was cloned from S. typhimurium LT7 and Escherichia coli B, and both genes were sequenced [2].
 

High impact information on cysJ

  • A plasmid containing cysJ gene, coding for the alpha subunit, overexpresses flavin reductase activity by 100-fold, showing that alpha is the site of free flavin reduction [3].
  • Characterization of the flavoprotein moieties of NADPH-sulfite reductase from Salmonella typhimurium and Escherichia coli. Physicochemical and catalytic properties, amino acid sequence deduced from DNA sequence of cysJ, and comparison with NADPH-cytochrome P-450 reductase [2].
  • Here we describe site-directed mutagenesis of the E. coli aroL, pncB, and cysJ coding sequences that was used to assess the contribution of naturally occurring adenines to in vivo expression and in vitro ribosome binding from mRNAs with different SD-containing untranslated leaders [4].
  • The cysJ promoter of Escherichia coli K-12, which is positively controlled by the CysB regulatory protein, was localized through the formation of a fusion of cysJ, the gene encoding NADPH-cytochrome c reductase with lacZ [1].
  • In vitro transcription initiation from the cysP promoter was shown to require both CysB protein and either O-acetyl-L-serine or N-acetyl-L-serine, which act as inducers, and was inhibited by the anti-inducer sulfide [5].
 

Biological context of cysJ

  • Primer extension analyses showed that the major in vivo transcription initiation site in S. typhimurium is located 171 nucleotides upstream of the cysJ start codon [6].
  • The cysP promoter of Salmonella typhimurium: characterization of two binding sites for CysB protein, studies of in vivo transcription initiation, and demonstration of the anti-inducer effects of thiosulfate [5].
  • Binding studies with fragments containing either CBS-P2 alone, CBS-P1 alone, or the entire cysP promoter region suggest a model in which the complex of bent DNA observed in the absence of O-acetyl-L-serine contains a single CysB protein molecule bound to CBS-P3 [5].
 

Associations of cysJ with chemical compounds

  • Paradoxically, in strains carrying plasmids containing the S. typhimurium cysJIH region, the highest levels of primer extension products were found with RNA from cells grown on L-cystine, even though levels of the proteins encoded by cysJ and cysI were normally repressed [6].
 

Other interactions of cysJ

  • Products of the cysJI genes were identified as the two subunits of NADPH-sulphite reductase (EC 1.8.1.2). cysJ encoded the flavoprotein, with > or = 39% identity to the protein from E. coli, and cysI encoded the haem protein, with > or = 53% identity [7].

References

  1. Mutagenesis and regulation of the cysJ promoter of Escherichia coli K-12. Loudon, J.A., Loughlin, R.E. Gene (1992) [Pubmed]
  2. Characterization of the flavoprotein moieties of NADPH-sulfite reductase from Salmonella typhimurium and Escherichia coli. Physicochemical and catalytic properties, amino acid sequence deduced from DNA sequence of cysJ, and comparison with NADPH-cytochrome P-450 reductase. Ostrowski, J., Barber, M.J., Rueger, D.C., Miller, B.E., Siegel, L.M., Kredich, N.M. J. Biol. Chem. (1989) [Pubmed]
  3. The flavin reductase activity of the flavoprotein component of sulfite reductase from Escherichia coli. A new model for the protein structure. Eschenbrenner, M., Covès, J., Fontecave, M. J. Biol. Chem. (1995) [Pubmed]
  4. Naturally Occurring Adenines within mRNA Coding Sequences Affect Ribosome Binding and Expression in Escherichia coli. Brock, J.E., Paz, R.L., Cottle, P., Janssen, G.R. J. Bacteriol. (2007) [Pubmed]
  5. The cysP promoter of Salmonella typhimurium: characterization of two binding sites for CysB protein, studies of in vivo transcription initiation, and demonstration of the anti-inducer effects of thiosulfate. Hryniewicz, M.M., Kredich, N.M. J. Bacteriol. (1991) [Pubmed]
  6. Molecular characterization of the cysJIH promoters of Salmonella typhimurium and Escherichia coli: regulation by cysB protein and N-acetyl-L-serine. Ostrowski, J., Kredich, N.M. J. Bacteriol. (1989) [Pubmed]
  7. Structure and function of a cysBJIH gene cluster in the purple sulphur bacterium Thiocapsa roseopersicina. Haverkamp, T., Schwenn, J.D. Microbiology (Reading, Engl.) (1999) [Pubmed]
 
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