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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Gene Review: 

ileX  -  tRNA

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3059, JWR0069
 
 
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Disease relevance of ileX

  • PCR analysis with primers specific for this analyzed region showed that in 11 Stx2-producing EHEC strains from patients with hemolytic uremic syndrome, all PCR-positive strains carried the ileX tRNA gene [1].
  • An ileX tRNA gene is located close to the Shiga toxin II operon in enterohemorrhagic Escherichia coli O157 and non-O157 strains [1].
 

High impact information on ileX

  • We isolated the gene for tRNA(2Ile) (ileX) and found that the anticodon is CAT, which is characteristic of the methionine tRNA gene [2].
  • Alternatively, the isoleucyl tRNA synthetase did not accumulate to detectable levels in cells grown in Luria broth unless the ileX tRNA was coexpressed when it accounted for 7 to 9% of total cell protein [3].
 

Analytical, diagnostic and therapeutic context of ileX

  • However, PCR analysis of the respective region in 11 Stxl-producing EHEC strains detected no ileX genes [1].

References

  1. An ileX tRNA gene is located close to the Shiga toxin II operon in enterohemorrhagic Escherichia coli O157 and non-O157 strains. Schmidt, H., Scheef, J., Janetzki-Mittmann, C., Datz, M., Karch, H. FEMS Microbiol. Lett. (1997) [Pubmed]
  2. Codon and amino-acid specificities of a transfer RNA are both converted by a single post-transcriptional modification. Muramatsu, T., Nishikawa, K., Nemoto, F., Kuchino, Y., Nishimura, S., Miyazawa, T., Yokoyama, S. Nature (1988) [Pubmed]
  3. Effects of a minor isoleucyl tRNA on heterologous protein translation in Escherichia coli. Del Tito, B.J., Ward, J.M., Hodgson, J., Gershater, C.J., Edwards, H., Wysocki, L.A., Watson, F.A., Sathe, G., Kane, J.F. J. Bacteriol. (1995) [Pubmed]
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