Disease relevance of aroB

• The dam-containing operon in Escherichia coli is located at 74 min on the chromosomal map and contains the genes aroK, aroB, a gene called urf74.3, dam and trpS [1].
• Cloning and characterisation of the Neisseria gonorrhoeae aroB gene [2].
• Cloning and analysis of the aroB gene encoding dehydroquinate synthase from Corynebacterium glutamicum [3].
• The aroB gene from Helicobacter pylori strain P1 was cloned and further characterized by sequence analysis and by functional complementation of the aroB mutation in Escherichia coli [4].
• The genomic sequences of the thermophiles Pyrococcus abyssi and Thermotoga maritima contain the aroG and aroB genes [5].

High impact information on aroB

• Three of the suppressors carried Tn5 in the aroK or aroB gene, the first two cistrons in the dam operon [6].
• The synthase is encoded by the aroB gene, which is carried by plasmid pLC29-47 from the Carbon-Clarke library [7].
• Complementation of the hemA-deficient mutant E. coli K-12 EB53 (aroB tsx malT hemA) with pap31 demonstrated that this protein is involved in heme acquisition and may be an important virulence factor in the pathogenesis of B. henselae [8].
• In this study, we cloned and sequenced a DNA fragment from an ordered cosmid library of Helicobacter pylori NCTC 11638 which confers to a siderophore synthesis mutant of Escherichia coli (EB53 aroB hemA) the ability to grow on iron-restrictive media and to reduce ferric iron [9].
• Here we describe the isolation of a cDNA encoding a 3-dehydroquinate synthase from tomato which was identified by complementing a 3-dehydroquinate synthase-deficient Escherichia coli strain with a tomato cDNA library [10].

Chemical compound and disease context of aroB

• Ferricrocinyl polyethylene glycol succinate (Mr 7000 -- 8500) promoted growth of E. coli K-12 AB2847 aroB under iron-limiting conditions [11].
• SitABCD mediated increased transport of both iron and manganese as demonstrated by uptake of 55Fe, 59Fe or 54Mn in E. coli K-12 strains deficient for the transport of iron (aroB feoB) and manganese (mntH) respectively [12].
• The first enzyme in the pathway, 3-deoxy-d-arabino-heptulosonic acid 7-phosphate synthase (aroG) and the second enzyme in the pathway, 5-dehydroquinic acid synthase (aroB) are "missing." The remaining five genes for the shikimate pathway in these organism are present and are similar to the corresponding Escherichia coli genes [5].

Biological context of aroB

• Analysis of the aroB nucleotide sequence and its 5'- and 3'-flanking regions has identified the aroB promoter elements and a possible 3'-terminator site [13].
• Three potential DnaA binding sites, each with homology of 8 of 9 bp, are present, two in the aroB promoter region and one just upstream of the dam gene [14].
• The defective gene is located between the crp and aroB genes at minute 73 on the E. coli chromosome [15].
• The complete amino acid sequence of the Escherichia coli 3-dehydroquinate synthase has been determined by a combined nucleotide and direct amino acid sequencing strategy [13].
• Complementation of the mutant with the intact aroB gene in trans indicates that the gene is responsible for the auxotrophic phenotype [2].

Associations of aroB with chemical compounds

• This 16 kDa open reading frame has been identified as aroK, the gene for shikimic acid kinase I. Thus the dam gene is part of an operon containing aroK, aroB, urf74.3, and dam [16].
• The predicted amino acid sequence of the H. pylori aroB gene product showed significant homology (30-40% identity and 50-60% similarity) to 3-dehydroquinate synthases from various other prokaryotes and eukaryotes [4].
• It suppressed the specific phenotype of aroB mutants by restoring the shikimate pathway-dependent synthesis of aromatic amino acids and the production of the siderophore enterobactin [4].
• A previously unreported inhibition of 3-dehydroquinate synthase by L-tyrosine was discovered to be a significant impediment to the flow of carbon through the common pathway [17].

Other interactions of aroB

• We present evidence for translational coupling between aroB and urf74.3 and also between rpe and gph [1].
• This observed linkage of the gerC genes with the ndk, aroC and aroB genes has been similarly observed in B. subtilis [18].
• Downstream of aroB a region of inverted repeats and a gene showing high homology to yafJ of E. coli has been identified [2].
• Further analysis revealed that the ftsD220 mapped at min 73 and that it is linked to cysG (6%) and to aroB (39%) [19].
• The presence of the aroB gene and the putative tgt homologue in unrelated H. pylori strains was confirmed by Southern blot hybridization and by polymerase chain reaction with specific primers [4].

Analytical, diagnostic and therapeutic context of aroB

• Restriction mapping and complementation analysis establish the gene order crp-nirB-cysG-aroB [20].

References