Disease relevance of gntR

• Three genes involved in gluconate metabolism, gntR, gntK, and gntU, which code for a regulatory protein, a gluconate kinase, and a gluconate transporter, respectively, were cloned from Escherichia coli K-12 on the basis of their known locations on the genomic restriction map [1].

High impact information on gntR

• Read-through transcription from the gntR promoter into gntK was decreased in the presence of GntR, although GntR did not repress its own promoter [2].
• CAT fusion analysis revealed that the operon has a promoter for gntR and another for gntKU, and that the gntR gene is constitutively expressed, while that of gntKU is regulated positively by the cAMP-CRP complex and negatively by GntR [2].
• Expression of gntR is essentially constitutive, while expression of gntKU is induced by gluconate and is subject to fourfold glucose catabolite repression [1].
• The gntR product is 331 amino acids long, with a helix-turn-helix motif typical of a regulatory protein [1].
• Our observations suggest that these loci might conform an operon distinct of gntT under the control of the gntR gene product [3].

References