Disease relevance of arsR

• Plasmid-determined arsenate resistance from plasmid pl258 encoded by genes arsR, arsB, and arsC was evolved in Escherichia coli [1].

High impact information on arsR

• The first gene in the operon, arsR, was previously shown to encode a homodimeric trans-acting metalloregulatory repressor protein [2].
• Fusions containing most of the arsR gene were still inducible by arsenicals [3].
• The arsR gene encodes the regulatory protein of the plasmid-encoded arsenical resistance operon [3].
• When cloned into a promoter probe vector this fragment conferred arsenite inducible tetracycline resistance in Escherichia coli, indicating that the fragment carried a regulatory gene, the arsR gene [4].
• A method was developed for monitoring antimonite and arsenite by using a single plasmid that incorporates the regulatory gene of the extrusion system, arsR, and the genes of bacterial luciferase, luxA and luxB [5].

Chemical compound and disease context of arsR

• An arsenite biosensor plasmid was constructed in Escherichia coli by inserting the operator/promoter region of the ars operon and the arsR gene from E. coli and the crtA gene, which is responsible for carotenoid synthesis in the photosynthetic bacterium, Rhodovulum sulfidophilum, into the broad-host-range plasmid vector, pRK415 [6].

Biological context of arsR

• The arsR gene, the first gene in this operon, was cloned using polymerase chain reaction [7].
• Plasmid pTOO31 was constructed by inserting the ars promoter and the arsR gene from plasmid R773 to control firefly luciferase gene expression [8].
• DNA sequencing revealed three open reading frames homologous to the arsR, arsB, and arsC open reading frames of plasmid-based arsenic resistance operons isolated from both E. coli and staphylococcal species [9].

Associations of arsR with chemical compounds

• Together with arsB and arsR, arsC participates in detoxification processes for the arsenate and arsenite ions [10].

References