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Gene Review

relB  -  antitoxin of the RelE-RelB toxin-antitoxin...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1558, JW1556, RC
 
 
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Disease relevance of relB

 

High impact information on relB

  • We have isolated a relB-complementing plasmid from the Clarke and Carbon E. coli DNA library [2].
  • A polypeptide of 9 kd compatible with this open reading frame was synthesized in maxicells and is in all probability the product of the relB gene [2].
  • We found that mutations in relB that conferred the delayed-relaxed phenotype destabilized RelB [3].
  • Later induction of transcription of the antitoxin genes relB or chpAI fully reversed the static condition induced by RelE and ChpAK respectively [4].
  • The direct interaction of the Escherichia coli cytotoxin RelE with its specific antidote, RelB, was demonstrated in two ways: (i) copurification of the two proteins and (ii) a positive yeast two-hybrid assay involving the relB and relE genes [5].
 

Biological context of relB

 

Associations of relB with chemical compounds

  • Genetic mapping studies indicate that the relaxed-control mutants isolated on the basis of sensitivity to glucose starvation contain lesions in the relB locus [8].
 

Other interactions of relB

  • However, expression of the relB gene counteracted the effect of relE to some extent, suggesting that toxin-antitoxin interaction also occurs in S. cerevisiae [1].
  • The phenotype of the relB mutants is accentuated by amber suppressors, and this effect is counteracted by a mutation, pus-1 that maps at 19.5 min near aroA [9].

References

  1. Bacterial toxin-antitoxin gene system as containment control in yeast cells. Kristoffersen, P., Jensen, G.B., Gerdes, K., Piskur, J. Appl. Environ. Microbiol. (2000) [Pubmed]
  2. Sequence of the relB transcription unit from Escherichia coli and identification of the relB gene. Bech, F.W., Jørgensen, S.T., Diderichsen, B., Karlström, O.H. EMBO J. (1985) [Pubmed]
  3. Delayed-relaxed response explained by hyperactivation of RelE. Christensen, S.K., Gerdes, K. Mol. Microbiol. (2004) [Pubmed]
  4. Rapid induction and reversal of a bacteriostatic condition by controlled expression of toxins and antitoxins. Pedersen, K., Christensen, S.K., Gerdes, K. Mol. Microbiol. (2002) [Pubmed]
  5. Purification of the RelB and RelE proteins of Escherichia coli: RelE binds to RelB and to ribosomes. Galvani, C., Terry, J., Ishiguro, E.E. J. Bacteriol. (2001) [Pubmed]
  6. The Escherichia coli relBE genes belong to a new toxin-antitoxin gene family. Gotfredsen, M., Gerdes, K. Mol. Microbiol. (1998) [Pubmed]
  7. Genetics of the relB locus in Escherichia coli. Diderichsen, B., Fiil, N.P., Lavallé, R. J. Bacteriol. (1977) [Pubmed]
  8. Evidence that glucose starvation-sensitive mutants are altered in the relB locus. Mosteller, R.D. J. Bacteriol. (1978) [Pubmed]
  9. Variations in phenotype of relB mutants of Escherichia coli and the effect of pus and sup mutations. Diderichsen, B., Desmarez, L. Mol. Gen. Genet. (1980) [Pubmed]
 
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