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Gene Review:

pfkA - 6-phosphofructokinase I

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3908, JW3887

Daldal, F. et al., Binet, M.R. et al., Thomson, J.A., Roehl, R.A. et al.

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Disease relevance of pfkA

Mutations in the genes pgi, pfkA, and ptsG resulted in a maltose Blu phenotype in Escherichia coli K-12, bringing the number of known Blu alleles to six [1].

High impact information on pfkA

Partial phenotypic suppression of the pfkA mutant phenotype results from Tn10 insertion very close to the pps gene, ca. 0.5 min from pgkB [2].
Strains deleted for both pfkA and pgkB are unable to grow at all on sugars whose primary route of metabolism is via fructose 6-phosphate, confirming earlier reports implicating the low Pfk-2 activity, rather than the pentose-phosphate pathway, as needed for the slow growth on sugars of pfkA pfkB+ strains [2].
A hybrid plasmid, pLC 16-4, from the ColE1-DNA (E. coli) bank of Clarke and Carbon (1976) carrying pfkA was used to program an in vitro protein synthesis system from E. coli [3].
These results suggest that in cells grown on fructose, fructose 1-phosphate could be converted to fructose 1,6-diphosphate either directly by the 1-PFK activity or via fructose 6-phosphate by the PFM and 6-phosphofructokinase activities [4].

References

New maltose Blu mutations in Escherichia coli K-12. Roehl, R.A., Vinopal, R.T. J. Bacteriol. (1979) [Pubmed]
Tn10 insertions in the pfkB region of Escherichia coli. Daldal, F., Fraenkel, D.G. J. Bacteriol. (1981) [Pubmed]
E. coli phosphofructokinase synthesized in vitro from a ColE1 hybrid plasmid. Thomson, J.A. Gene (1977) [Pubmed]
Fructose and mannose metabolism in Aeromonas hydrophila: identification of transport systems and catabolic pathways. Binet, M.R., Rager, M.N., Bouvet, O.M. Microbiology (Reading, Engl.) (1998) [Pubmed]

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