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Gene Review

metF  -  5,10-methylenetetrahydrofolate reductase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3933, JW3913
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Disease relevance of metF


High impact information on metF


Biological context of metF

  • The nucleotide sequence of the E.coli metF gene (888 nucleotides), coding for 5-10 methylene tetrahydrofolate reductase, has been determined [1].
  • The introduction of new restriction sites within the metJBLF gene cluster due to the Tn5 insertion events allowed the metF transcription unit to be cloned into the high copy number plasmid pBR322 [5].
  • Two of these mutants, lambda dmet128::Tn5, MW77 and lambda dmet128::Tn5, 3-1, were used to locate precisely as well as confirm the existence of the metF transcription unit (approximately 1,000 base pairs in size) [5].

Associations of metF with chemical compounds

  • Expression of the plasmid borne metF allele in cells grown in the absence, or presence, of exogenous L methionine (0.2 mM) demonstrates that the amplification of the metF copy number does not abolish met regulon mediated control of the gene's activity [5].


  1. Nucleotide sequence of metF, the E. coli structural gene for 5-10 methylene tetrahydrofolate reductase and of its control region. Saint-Girons, I., Duchange, N., Zakin, M.M., Park, I., Margarita, D., Ferrara, P., Cohen, G.N. Nucleic Acids Res. (1983) [Pubmed]
  2. Regulation of the Salmonella typhimurium metF gene by the MetR protein. Cowan, J.M., Urbanowski, M.L., Talmi, M., Stauffer, G.V. J. Bacteriol. (1993) [Pubmed]
  3. Salmonella typhimurium LT2 metF operator mutations. Stauffer, G.V., Stauffer, L.T. Mol. Gen. Genet. (1988) [Pubmed]
  4. The Escherichia coli regulatory protein MetJ binds to a tandemly repeated 8 bp palindrome. Davidson, B.E., Saint Girons, I. Mol. Microbiol. (1989) [Pubmed]
  5. Mutagenesis of the metJBLF gene cluster with transposon Tn5: localization of the metF transcription unit. Treat, M.L., Weaver, M.L., Emmett, M.R., Johnson, J.R. Mol. Gen. Genet. (1984) [Pubmed]
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