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Gene Review:

phnD - phosphonate ABC transporter periplasmic...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK4098, JW4066, psiD

Wanner, B.L. et al., Rizk, S.S. et al., Metcalf, W.W. et al., Metcalf, W.W. et al., Chen, C.M. et al., et al.

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Disease relevance of phnD

Molecular biology of carbon-phosphorus bond cleavage. Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B [1].

High impact information on phnD

Here we report the characterization of the phosphonate-binding properties of the phnD protein product [2].
The Escherichia coli phn (psiD) locus consists of a large gene cluster encoding proteins necessary for the use of phosphonates (Pn) as a sole phosphorus source [3].
The Escherichia coli phn (psiD) locus encodes genes for phosphonate (Pn) utilization, for phn (psiD) mutations abolish the ability to use as a sole P source a Pn with a substituted C-2 or unsubstituted hydrocarbon group such as 2-aminoethylphosphonate (AEPn) or methylphosphonate (MPn), respectively [4].
Also, Mu d1s in the phoA and phn (psiD) loci of the phosphate regulon showed regional specificities for the insertion sites despite the randomness of Mu d1 insertions into the genome as a whole [5].
The phn(EcoB) DNA hybridized to overlapping lambda clones in the E. coli K-12 gene library (Y. Kohara, K. Akiyama, and K. Isono, Cell 50:495-508, 1987) which contain the 93-min region, thus showing that the phn (psiD) locus was itself cloned and verifying our genetic data on its map location [4].

Chemical compound and disease context of phnD

Involvement of the Escherichia coli phn (psiD) gene cluster in assimilation of phosphorus in the form of phosphonates, phosphite, Pi esters, and Pi [6].
The Escherichia coli phnD gene is hypothesized to code for the periplasmic binding component of a phosphonate uptake system [2].

Biological context of phnD

The phn (psiD) gene cluster is induced during Pi limitation and is required for the use of phosphonates (Pn) as a phosphorus (P) source [6].

Associations of phnD with chemical compounds

Since psiD::lacZ(Mu d1) mutants cannot metabolize methylphosphonic acid, psiD may be the structural gene(s) for C-P lyase [7].

Analytical, diagnostic and therapeutic context of phnD

Identification of cognate ligands for the Escherichia coli phnD protein product and engineering of a reagentless fluorescent biosensor for phosphonates [2].
Mapping and molecular cloning of the phn (psiD) locus for phosphonate utilization in Escherichia coli [4].

References

Molecular biology of carbon-phosphorus bond cleavage. Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B. Chen, C.M., Ye, Q.Z., Zhu, Z.M., Wanner, B.L., Walsh, C.T. J. Biol. Chem. (1990) [Pubmed]
Identification of cognate ligands for the Escherichia coli phnD protein product and engineering of a reagentless fluorescent biosensor for phosphonates. Rizk, S.S., Cuneo, M.J., Hellinga, H.W. Protein Sci. (2006) [Pubmed]
Evidence for a fourteen-gene, phnC to phnP locus for phosphonate metabolism in Escherichia coli. Metcalf, W.W., Wanner, B.L. Gene (1993) [Pubmed]
Mapping and molecular cloning of the phn (psiD) locus for phosphonate utilization in Escherichia coli. Wanner, B.L., Boline, J.A. J. Bacteriol. (1990) [Pubmed]
Identification of phosphate starvation-inducible genes in Escherichia coli K-12 by DNA sequence analysis of psi::lacZ(Mu d1) transcriptional fusions. Metcalf, W.W., Steed, P.M., Wanner, B.L. J. Bacteriol. (1990) [Pubmed]
Involvement of the Escherichia coli phn (psiD) gene cluster in assimilation of phosphorus in the form of phosphonates, phosphite, Pi esters, and Pi. Metcalf, W.W., Wanner, B.L. J. Bacteriol. (1991) [Pubmed]
Involvement of the phosphate regulon and the psiD locus in carbon-phosphorus lyase activity of Escherichia coli K-12. Wackett, L.P., Wanner, B.L., Venditti, C.P., Walsh, C.T. J. Bacteriol. (1987) [Pubmed]

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