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Gene Review

pyrI  -  aspartate carbamoyltransferase, regulatory...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK4239, JW4203
 
 
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Disease relevance of pyrI

  • Structure-function relationship in allosteric aspartate carbamoyltransferase from Escherichia coli. I. Primary structure of a pyrI gene encoding a modified regulatory subunit [1].
  • We now report the sequence of the mutated pyrI and show that, during the generation of this pyrBI-bearing phage, six codons from lambda DNA have been substituted for the eight terminal codons of the wild-type gene [1].
  • The genes encoding the catalytic (pyrB) and regulatory (pyrI) polypeptides of aspartate transcarbamoylase (ATCase, EC 2.1.3.2) from several members of the family Enterobacteriaceae appear to be organized as bicistronic operons [2].
 

High impact information on pyrI

  • In this deletion mutation, there is no production of the regulatory polypeptide, indicating that a single region adjacent to pyrB controls transcription of pyrI as well [3].
  • The cistrons are separated by a 15-nucleotide untranslated region containing a sequence capable of interacting with the 16S ribosomal RNA and allowing translation of the pyrI cistron [3].
  • A model is presented for the formation of this modified pyrI gene during the integrative recombination of the parental lambda phage with the Escherichia coli chromosome [1].
  • The two genes were found to constitute an operon, with pyrI being the distal gene and separated from pyrB by a 15-bp intercistronic region [4].
 

Biological context of pyrI

  • Evidence for this single transcriptional unit was obtained by a study of various deletion mutations and from the DNA sequence of the intercistronic region between pyrB and pyrI [3].
 

Other interactions of pyrI

 

Analytical, diagnostic and therapeutic context of pyrI

References

  1. Structure-function relationship in allosteric aspartate carbamoyltransferase from Escherichia coli. I. Primary structure of a pyrI gene encoding a modified regulatory subunit. Cunin, R., Jacobs, A., Charlier, D., Crabeel, M., Hervé, G., Glansdorff, N., Piérard, A. J. Mol. Biol. (1985) [Pubmed]
  2. In vivo formation of hybrid aspartate transcarbamoylases from native subunits of divergent members of the family Enterobacteriaceae. Foltermann, K.F., Beck, D.A., Wild, J.R. J. Bacteriol. (1986) [Pubmed]
  3. Genes encoding Escherichia coli aspartate transcarbamoylase: the pyrB-pyrI operon. Pauza, C.D., Karels, M.J., Navre, M., Schachman, H.K. Proc. Natl. Acad. Sci. U.S.A. (1982) [Pubmed]
  4. Cloning, nucleotide sequence and expression of the pyrBI operon of Salmonella typhimurium LT2. Michaels, G., Kelln, R.A., Nargang, F.E. Eur. J. Biochem. (1987) [Pubmed]
 
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