Disease relevance of yicR

• Molecular cloning and DNA sequencing of the radC gene of Escherichia coli K-12 [1].
• Molecular analysis and identification of the radC gene from the phototrophic bacterium Rhodobacter capsulatus B10 [2].

High impact information on yicR

• A low-copy-number plasmid carrying the radC(+) gene did not affect the UV sensitivity of a wild-type strain, a radC102 strain, or a recG258::Tn10mini-kan strain [3].
• This operon has transcription initiation sites upstream of radC, in the radC coding region, and immediately upstream of fpg [4].
• Though the radC mutant shows deficient excision repair, it can remove 6-4 photoproducts to a moderate degree [5].
• The proximity of the radC gene to this insertion mutation provided a strategy to isolate the radC+ gene based on the cloning of radC+ and fpg-1::KnR on the same DNA fragment using the resistance to kanamycin as a selector [1].
• Escherichia coli radC is deficient in the recA-dependent repair of X-ray-induced DNA strand breaks [6].

Biological context of yicR

• 1. The DNA sequence of the radC gene suggests an open reading frame of 297-bp [7].
• For cells grown in rich medium, the radC mutant was normal for gamma-radiation mutagenesis, but showed less uv-radiation mutagenesis than the wild-type strain; it showed normal amounts of X- and uv-radiation-induced DNA degradation, and it was approximately 60% deficient in recombination ability [8].
• The radC gene, whose product plays a role in the prokaryotic repair of DNA damage after UV and X-ray irradiation, was cloned and sequenced from the phototrophic bacterium Rhodobacter capsulatus B10 [2].

Associations of yicR with chemical compounds

• In studies using neutral sucrose gradients, the radC strain consistently showed a small deficiency in rejoining X-ray-induced DNA double-strand breaks, and it was deficient in restoring the normal sedimentation characteristics of the repaired DNA [6].

Analytical, diagnostic and therapeutic context of yicR

• We examined the removal of cyclobutane pyrimidine dimers (CPD) and 6-4 photoproducts from DNA in the radC mutant and the wild-type strain using an enzyme-linked immunosorbent assay with monoclonal antibodies [5].
• Northern blot analysis indicated that under both chemotrophic and phototrophic growth conditions the radC gene was relatively highly expressed and was induced about five-fold after UV-irradiation [2].

References