Disease relevance of cro

• A2 cro, the lysogenic cycle repressor, specifically binds to the genetic switch region of Lactobacillus casei bacteriophage A2 [1].
• Consequently, Cro abolishes the binding of sigma(A)-RNAP to the genetic switch of A2 and, presumably, its own synthesis, contributing indirectly to the entry of phage development into its late stages [2].

High impact information on cro

• These promoters mediate transcription from cI and a putative cro, respectively [3].
• CI selectively interacts with O1, which is placed downstream from the transcription start point of the cro gene, and with O2 and O3, which overlap with the -35 region of the two promoters [3].
• Interaction of the Cro repressor with the lysis/lysogeny switch of the Lactobacillus casei temperate bacteriophage A2 [2].
• A Cro mutant protein, devoid of the last 12 residues (Cro*), allowed the assignment of the DNA-binding domain to the NH2 end of Cro [1].
• Adjacent, but divergently transcribed, lies another open reading frame whose product resembles the lambda Cro protein [4].

Biological context of cro

• Lysogenic induction of temperate bacteriophage A2 of Lactobacillus casei is controlled by the action of its cI and cro products at the phage operator region [1].

Analytical, diagnostic and therapeutic context of cro

• The binding of Cro to the three operator subsites is noncooperative and yields two discrete protein-DNA complexes of retarded migration in mobility shift assays [1].

References