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Gene Review:

prpD - 2-methylcitrate dehydratase

Escherichia coli O157:H7 str. EDL933

Lee, S.K. et al., Brämer, C.O. et al.

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Disease relevance of prpD

The expression and regulatory properties of a propionate-regulated overexpression system (Salmonella enterica prpBCDE promoter (P(prpB)) and positive regulator (prpR)) were evaluated in wild-type S. enterica serovar Typhimurium TR6583 and prpB(-) or prpD(-) versions of this strain and compared with the arabinose-regulated T7 expression system [1].

High impact information on prpD

The prpD(-) strain exhibited low expression in the presence of glucose, highly regulatable expression over a wide range of propionate concentrations, and, in contrast to the wild-type strain, fast induction to full expression under all growth conditions [1].
In contrast, inactivation of prpD did not block the ability of the cell to use propionic acid as carbon and energy source, as shown by the propionic acid phenotype of a null-allele mutant [2].

References

A Salmonella-based, propionate-inducible, expression system for Salmonella enterica. Lee, S.K., Keasling, J.D. Gene (2006) [Pubmed]
The methylcitric acid pathway in Ralstonia eutropha: new genes identified involved in propionate metabolism. Brämer, C.O., Steinbüchel, A. Microbiology (Reading, Engl.) (2001) [Pubmed]

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