Disease relevance of uraA

• The BCG OMP-DCase gene (uraA) and the flanking DNA were sequenced [1].
• The uraA locus and homologous recombination in Mycobacterium bovis BCG [1].
• In addition, for1-1/for1-1 plants were restored to FOA sensitivity by transformation with the Escherichia coli uracil transporter gene uraA driven by the cauliflower mosaic virus (CaMV) 35S promoter [2].

High impact information on uraA

• A second open reading frame was found upstream of uraA and oriented in the same direction as uraA [3].
• To investigate whether homologous integration can occur in M. bovis BCG, an improved protocol for transformation of BCG was developed and a linear fragment of mycobacterial DNA containing the uraA locus, marked with a kanamycin resistance gene, was introduced into BCG cells by electroporation [1].
• Uridine and uracil transport in Escherichia coli and transport-deficient mutants [4].

Biological context of uraA

• A stretch of 21 consecutive pyrimidine (C or T) residues were found in the intercistronic region between the potential ribosome-binding site of uraA and the UGA stop codon of the upstream open reading frame [3].

References