Disease relevance of nrfA

• Independent regulation of the divergent Escherichia coli nrfA and acsP1 promoters by a nucleoprotein assembly at a shared regulatory region [1].

High impact information on nrfA

• A null mutant, constructed by disruption of nrfA, exhibited the same phenotype as the missense mutant [2].
• Inactivation of nrfA in the mutant was due to a missense mutation resulting in the replacement of a cysteine residue by arginine [2].
• The nrfHAIJ locus was restored on the genome of the W. succinogenes DeltanrfAIJ deletion mutant by integration of a plasmid, thus enabling the expression of modified alleles of nrfA and nrfI [3].
• The structural genes nrfA and nrfH were identified in an apparent operon (nrfHAIJ) with two additional genes [4].
• Under these conditions, the presence of high levels of nitrate suppressed nrfA gene expression [5].

Chemical compound and disease context of nrfA

• The nrfA and nirB nitrite reductase operons in Escherichia coli are expressed differently in response to nitrate than to nitrite [5].

Associations of nrfA with chemical compounds

• Lastly, since nrfA operon expression is only induced under low-nitrate concentrations, the NrfA enzyme is predicted to have a physiological role only where nitrate (or nitrite) is limiting in the cell environment [5].

Analytical, diagnostic and therapeutic context of nrfA

• Gel retardation assays were used to investigate the pairwise binding of FNR, NarL, Fis and IHF proteins to the nrfA-acs intergenic region [1].

References