Disease relevance of glgC

• Cloning and expression of the Escherichia coli glgC gene from a mutant containing an ADPglucose pyrophosphorylase with altered allosteric properties [1].

High impact information on glgC

• CsrA is a global regulator that binds to two sites in the glgCAP leader transcript, thereby blocking ribosome access to the glgC Shine-Dalgarno sequence [2].
• A purified recombinant CsrA protein was found to inhibit in vitro glg (glycogen biosynthesis) gene expression posttranscriptionally and bind specifically to a glgC runoff transcript without causing its decay [3].
• The csrA gene encodes a global regulatory protein which facilitates glgC mRNA decay in vivo [3].
• The region between glgB and glgC contains an open reading frame (ORF) of 1521 bp which we call glgX [4].
• The nucleotide sequences of the Escherichia coli genome between the glycogen biosynthetic genes glgB and glgC, and 1170 bp of DNA which follows glgA have been determined [4].

Biological context of glgC

• The structural gene, glgC, for this allosteric mutant enzyme was cloned into the bacterial plasmid pBR322 by inserting the chromosomal DNA at the PstI site [1].

References