Disease relevance of PTDSS1

• Precision of a patient-weighted symptom score in prostatism. The DAN-PSS-1 questionnaire [1].

High impact information on PTDSS1

• Control of phosphatidylserine biosynthesis through phosphatidylserine-mediated inhibition of phosphatidylserine synthase I in Chinese hamster ovary cells [2].
• In the present study, the PtdSer-resistant PtdSer biosynthesis in the mutant was traced to a point mutation in the PtdSer synthase I gene, pssA, resulting in the replacement of Arg-95 of the synthase by lysine [2].
• In situ hybridization studies indicate differential expression of the PSS1 and PSS2 genes in the septum, the lateral part of the pallium, the amygdaloid complex, the posterior nuclei of the thalamus, the ventral hypothalamic nucleus, the torus semicircularis and the optic tectum [3].
• A phosphatidylserine-auxotrophic mutant of cultured Chinese hamster ovary (CHO) cells, PSA-3, is defective in phosphatidylserine synthase I activity [4].
• Transfection of PSA-3 cells with a cDNA expression library of CHO-K1 (the parent of PSA-3) yielded a phosphatidylserine-prototrophic transformant with normal phosphatidylserine synthase I activity [4].

Biological context of PTDSS1

• The activities of CTP : phosphocholine cytidylyltransferase (CCT) and phosphatidylserine synthase were also reduced in brain tissue from Hexb-/- mice, probably because of post-translational modification as no changes were observed in levels of enzyme expression [5].
• Externalization of phosphatidylserine during apoptosis does not specifically require either isoform of phosphatidylserine synthase [6].

Anatomical context of PTDSS1

• The mitochondria-associated membranes are enriched in lipid biosynthetic enzymes, especially phosphatidylserine synthase [7].
• The reason for the greater synthesis of PS may be the higher level of expression of PSS1 in immature oocytes [8].
• Activities of two additional phospholipid biosynthetic enzymes-CTP:phosphocholine cytidylyltransferase and phosphatidylserine synthase-were also reduced by > 50% in mnd/mnd microsomes [9].
• CONCLUSIONS: The DAN-PSS-1 system is reliable, valid and responsive, and therefore can be recommended for assessing the severity of symptoms among patients presenting with lower urinary tract complaints suggestive of BPH and in the follow-up after intervention [10].
• In all cell lines, regardless of their content of PSS1 and/or PSS2, apoptosis occurred to approximately the same extent, and within approximately the same time frame, as in parental CHO-K1 cells [6].

Associations of PTDSS1 with chemical compounds

• Introduction of the pssA cDNA into PSA-3 cells restored the phosphatidylserine content, and the resultant transformant exhibited 15-fold higher specific phosphatidylserine synthase I activity than CHO-K1 cells [4].

Other interactions of PTDSS1

• Phosphatidylserine synthase is found predominantly in the microsomal fraction, and phosphatidylserine decarboxylase is found predominantly in the mitochondrial fraction of baby hamster kidney (BHK-21) cells [11].
• All three species contained phosphatidate cytidylyltransferase and phosphatidylserine synthase [12].
• Finally, the two base-exchange enzymes, phosphatidylserine synthase-1 and phosphatidylserine synthase-2, that synthesize phosphatidylserine in mammalian cells are also discussed [13].

Analytical, diagnostic and therapeutic context of PTDSS1

• The distribution of PSS1- and PSS2-derived peptides has been investigated by immunohistochemistry using two antisera directed against SS1 and the sequence 54-66 of PSS2 (PSS2(54-66)), respectively [14].
• Following TURP, the DAN-PSS-1 score was reduced by 80% after 6-10 weeks (visit 2) and by 100% after 12-16 weeks (visit 3) [15].
• Activities of CTP:ethanolaminephosphate cytidyltransferase (EC 2.7.7.14), phosphatidylserine decarboxylase (EC 4.1.1.65) and phosphatidylserine synthase; CDP-DAG:L-serine o-phosphatidyltransferase (EC 2.7.8.8) were measured and additionally, the presence of a PS decarboxylase (PSD1) in oat was confirmed by immunoblotting [16].

References