Disease relevance of Plastic Embedding

• This fixative stabilized nucleic acids, allowing acetone dehydration and plastic embedding without collapse of nucleic acid to the centre of the virion [1].

High impact information on Plastic Embedding

• A simplified three-step plastic embedding system using acetone-fixed tissues embedded in glycol-methacrylate (GMA) resin has been found to provide both excellent morphologic and antigenic preservation [2].
• A simplified plastic embedding and immunohistologic technique for immunophenotypic analysis of human hematopoietic and lymphoid tissues [2].
• Anisotropy ratios of mineralized tissues were not affected by formalin fixation or plastic embedding [3].
• Plastic embedding of cutaneous specimens for light microscopy using araldite epoxy resin [4].
• We demonstrate 2DG emulsion autoradiography combined with cytochrome oxidase (CO) histochemistry, markers for axonal pathway tracing, plastic embedding for semithin sections, and immunohistochemical staining for glutamate decarboxylase (GAD) [5].

Chemical compound and disease context of Plastic Embedding

• To avoid the possible loss or the artifactual redistribution of the phospholipid occurring during dehydration with organic solvents and plastic embedding, the fixed specimens were embedded in aqueous albumin, which was then hardened by glutaraldehyde and dehydrated physically [6].

Biological context of Plastic Embedding

• This study reveals the possibility of complementing immunocytochemical studies of extracellular matrix with enzyme cytochemistry and, above all, with the excellent tissue preservation and high resolution afforded by plastic embedding [7].

Anatomical context of Plastic Embedding

• This study utilized improved methods of fixation and plastic embedding to quantitatively evaluate the effects of gossypol on germ cells and Leydig cells in testes of rats made infertile with gossypol [8].
• After glutaraldehyde fixation and treatment with ethanolic phosphotungstic acid (E-PTA) before plastic embedding, sections of rat large intestine showed a characteristic electron contrasting pattern in epithelial cells [9].
• Quantitative analysis of human seminiferous epithelium was carried out using an improved method of glutaraldehyde and osmium fixation with plastic embedding [10].

Associations of Plastic Embedding with chemical compounds

• To study the possible role of plastic embedding in the surgical pathologic diagnosis of Pneumocystis carinii pneumonia, bronchial biopsy specimens from five patients with AIDS were examined using 0.9 micron thick methacrylate sections stained with silver methenamine borate and counterstained with toluidine blue [11].
• Acidified 2,2-dimethoxypropane (DMP) has been used as a rapid chemical dehydrating agent en route to plastic embedding and ulthrathin sectioning for conventional electron miscroscopy (J Histochem Cytochem 23:107, 1975) [12].
• After cryoultramicrotomy, however, even after osmium fixation, antibody may be used 1000 times more diluted than after plastic embedding [13].
• For light microscopy both plastic embedding systems: methylmethacrylate (MMA) and glycolmethacrylate (GMA) can be stained with this technique [14].
• Plastic embedding. Use of glycol methacrylate-embedded biopsy material at the light microscopic level [15].

Gene context of Plastic Embedding

• Conventional plastic embedding as well as hydrophilic media (L.R. White) were used; non-osmicated and osmicated tissues were immunolabeled; sodium metaperiodate oxidation was used, but was not essential for immunolabeling [16].
• We report a simple glycol-methacrylate (GMA) embedding technique using vibratome-sectioned mouse brain reacted for tyrosine hydroxylase (TH) immunoreactivity before plastic embedding [17].

References