Disease relevance of False Negative Reactions

• Light-chain proteinuria: spurious false-negative reaction to sulfosalicylic acid [1].
• Different factors in everyday practice, including autolysis and technical problems often produce false negative reactions with the result that FN cannot be regarded as a reliable parameter of vitality [2].

High impact information on False Negative Reactions

• False positive and false negative reactions encountered in the use of sulfite test strips for the detection of sulfite-treated foods [3].
• Among 40 selected sera, 8 false-negative reactions were observed in P-S ELISA [4].
• To detect the presence of inhibitors of the Taq polymerase, which may cause false-negative reactions, part of each sample was spiked with M. tuberculosis DNA [5].
• Two of the false-negative reactions occurring with the CAS were with GT-negative strains of C. albicans [6].
• False-negative reactions due to proteases present in the stool samples were avoided by the addition of phenylmethylsulfonyl fluoride [7].

Associations of False Negative Reactions with chemical compounds

• The role of aluminium in the false-negative reactions is discussed [8].
• For other corticosteroids, the use of ethanol as a vehicle resulted in both false-positive and false-negative reactions [9].
• The concentration of 0.1% methyldibromoglutaronitrile in the currently available commercial allergens appears to be too low, resulting in a number of false-negative reactions [10].
• False-negative reactions were apparent in the pigs at halothane concentrations less than 3% [11].
• The 2% NiCl2 hydrogel produced a small increase in response frequency and may reduce false-negative reactions [12].

Gene context of False Negative Reactions

• Because the false-negative reactions given by the Ca series of antibodies are to some extent complementary to those given by monoclonal antibodies directed against the carcinoembryonic antigen (CEA), a combination of Ca2 and anti-CEA is recommended as a most useful addition to the normal cytologic examination of effusions [13].
• The data for these sera indicate that in some cases the standard IP assay gives false-negative reactions [14].
• Whereas both false-positive and false-negative reactions were obtained when absorbed CFA antisera were used for agglutination, antisera against purified CFAs were equally effective as immunodiffusion in identifying CFA/I and CFA/II-carrying strains [15].
• Responses to TSA however were highly specific, with no false negative reactions [16].
• In both test versions HCG concentrations up to 200,000 IU/1 did not result in false negative reactions [17].

Analytical, diagnostic and therapeutic context of False Negative Reactions

• False positives occurred with sera from seven hemophiliacs that were negative on Western blots, and false-negative reactions were observed on two occasions [18].
• Although "false positive" reactions were few (positive crossmatch but satisfactory transfusion response), "false negative" reactions (negative crossmatch but poor transfusion response) were unacceptably high (43% by lymphocytotoxicity, 60% by serotonin release, 76% by platelet factor 3 release, and 83% by aggregometry) [19].
• The first case may be explained by a compound allergy or by a false-negative reaction to calcipotriol due to the very low concentration used for patch tests [20].

References