High impact information on Skin, Artificial

• In coculture and artificial skin equivalent experiments, subsets of T-regulatory cells neither induced keratinocyte death nor suppressed apoptosis induced by skin T cells, T(H)1 cells, IFN-gamma, or TNF-alpha [1].
• Purified EGFP-positive keratinocytes grafted as part of the fibrin-based artificial skin were capable of generating multilayer human epidermis on mice, with well-developed granulosum and corneum strata, and clearly defined rete ridges [2].
• Local expression of indoleamine 2,3-dioxygenase protects engraftment of xenogeneic skin substitute [3].
• Cultured skin substitute inserts were evaluated at 2 and 5 wk for viability by incorporation of 5-bromo-2'-deoxyuridine (BrdU) and by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) conversion [4].
• An approximately 2-fold decrease in staining for Ki67, a proliferation marker, was observed with meshing of human bilayered skin substitute [5].

Biological context of Skin, Artificial

• These results indicate that genetic modification of keratinocytes in cultured skin substitutes can lead to increased vascular endothelial growth factor expression, which could prospectively improve vascularization of cultured skin substitutes for wound healing applications [6].
• As a possible way of making a non-rejectable skin substitute, here, we ask the question of whether the expression of indoleamine 2,3-dioxygenase (IDO) selectively suppresses immune, but skin, cell proliferation [7].
• Flexibilized gelatin film-based artificial skin model: II. Release kinetics of incorporated bioactive molecules [8].

Anatomical context of Skin, Artificial

• Therefore, the aggregates could provide an alternative method for seeding fibroblasts to scaffold for an artificial skin, such as a mesh of polyglycolic acid [9].
• Preliminary assessment of alginic acid as a factor buffering triethanolamine interacting with artificial skin sebum [10].
• Vascular endothelial cells, which were identified using alkaline phosphatase stain, were significantly increased in the fibrin-type cultured skin substitute [11].

Associations of Skin, Artificial with chemical compounds

• Effects of all-trans retinoic acid on UVB-irradiated human skin substitute [12].
• To address this hypothesis, vitamin C was titrated at 0.0, 0.01, 0.1, and 1.0 mM in a cultured skin substitute model on filter inserts [4].
• Semipermeable polyurethane membrane as an artificial skin for the premature neonate [13].
• These results, in combination with other preliminary findings for biocompatibility and fatigue behavior, suggest that PVA hydrogel is a promising biomaterial for implants, catheters, and artificial skin [14].
• Design of an artificial skin. II. Control of chemical composition [15].

Gene context of Skin, Artificial

• Here, we report the possible use of this enzyme to suppress proliferation of immune cells cocultured with IDO-expressing fibroblasts of an allogenic skin substitute [16].
• Expression of interleukin-1alpha, interleukin-6, and basic fibroblast growth factor by cultured skin substitutes before and after grafting to full-thickness wounds in athymic mice [17].
• Expression of human beta-defensins HBD-1, HBD-2, and HBD-3 in cultured keratinocytes and skin substitutes [18].
• These data suggest that genetically modified skin substitutes secreting HGF may have applications in wound closure and the promotion of wound healing [19].
• However, it remains unknown whether VEGF is induced when fibrin is used as a dermal substrate for cultured skin substitutes [11].

Analytical, diagnostic and therapeutic context of Skin, Artificial

• A blinded study was designed to provide qualitative and quantitative evaluation on the possible therapeutic benefits of the use of Pluronic Polyol F-127 as a substitute skin in standardized third-degree thermal burns [20].
• A study on a chitosan-gelatin-hyaluronic acid scaffold as artificial skin in vitro and its tissue engineering applications [21].
• Experiments were performed using 12 cultured skin substitutes: four for histologic examination before transplantation, four for VEGF assay in vitro, and four for the transplantation to athymic mice [11].
• This observation is very important because retroviral vectors of genes, such as platelet derived growth factor (PDGF) or vascular endothelial growth factor (VEGF), are expected to facilitate the implementation of these modified cultures for tissue grafting and skin substitute development and potentiate the effectiveness of the grafts [22].

References