Disease relevance of Xylella

• GyrA (Leu-55 to Gln-155, Escherichia coli numbering), GyrB (Met-391 to Phe-513), ParC (Ile-34 to Arg-124), and ParE (Leu-396 to Leu-567) fragments from strain ATCC 13637 showed high degrees of identity to the corresponding regions from the phytopathogen Xylella fastidiosa, with the degrees of identity ranging from 85.0 to 93.5% [1].
• In this study, an efficient expression system, based on the pET32Xa/LIC vector, for producing a Xylella fastidiosa virulence-associated protein D, found to have a strong similarity to Riemerella anatipestifer and Actinobacillus actinomycetencomitans VapD protein, is presented [2].
• RESULTS: This work uses genome in silico analysis to propose methionine salvage pathways for Klebsiella pneumoniae, Leptospira interrogans, Thermoanaerobacter tengcongensis and Xylella fastidiosa [3].
• The availability of the genome sequence of the bacterial plant pathogen Xylella fastidiosa, the causal agent of citrus variegated chlorosis, is accelerating important investigations concerning its pathogenicity [4].

High impact information on Xylella

• A genomic approach to the understanding of Xylella fastidiosa pathogenicity [5].
• Upstream migration of Xylella fastidiosa via pilus-driven twitching motility [6].
• Detection and diversity assessment of Xylella fastidiosa in field-collected plant and insect samples by using 16S rRNA and gyrB sequences [7].
• These genes are conserved in order with other eubacterial oriC genes and code for proteins that share high degrees of identity with their homologues, except for orf900, which has a homologue only in Xylella fastidiosa [8].
• Mutagenesis by homologous recombination was evaluated in Xylella fastidiosa by using the bga gene, coding for beta-galactosidase, as a model [9].

Chemical compound and disease context of Xylella

• Molecular models for shikimate pathway enzymes of Xylella fastidiosa [10].
• The GumH enzyme from Xylella fastidiosa catalyzes the transfer reaction of a mannose from GDP-mannose to the carrier lipid cellobiose-pyrophosphate-polyprenol (Glc(2)-PP-Lip), an intermediary in the reaction for the synthesis of the exopolysaccharide (EPS) fastidian gum [11].
• Xylella fastidiosa organic hydroperoxide-resistance protein (Ohr) is a dithiol-dependent peroxidase that is widely conserved in several pathogenic bacteria with high affinity for organic hydroperoxides [12].
• A simple defined solid medium containing citrate and succinate, three amino acids (L-glutamine, L-asparagine, and L-cysteine), hemin chloride, potato starch, gellan gum (GelRite), and mineral salts supported the growth of grape strains of Xylella fastidiosa, the bacterial pathogen that causes Pierce's disease of grape [13].
• Whole-genome expression profiling of Xylella fastidiosa in response to growth on glucose [14].

Biological context of Xylella

• Transposon mutagenesis of Xylella fastidiosa by electroporation of Tn5 synaptic complexes [15].
• Analysis of gene expression in two growth states of Xylella fastidiosa and its relationship with pathogenicity [16].
• Construction of a shuttle vector and transformation of Xylella fastidiosa with plasmid DNA [17].

Gene context of Xylella

• Cloning, expression, and purification of the virulence-associated protein D from Xylella fastidiosa [2].
• Transcription analysis of pilS and xpsEL genes from Xylella fastidiosa [18].
• Xylella fastidiosa gumB and gumF mutants exhibited normal cell characteristics; typical colony morphology and EPS biosynthesis were not altered [19].
• When a DNA fragment encompassing part of the Xylella xpsD gene was cloned into pSP3, specific integration of the construct into this gene was observed in 10% of the transformants, as early as after two passages of the culture [20].
• The organization of these transfer genes in pIPO2 is highly similar to the genetic organization seen in the environmental plasmid pSB102 and in pXF51 from the plant pathogen Xylella fastidiosa [21].

Analytical, diagnostic and therapeutic context of Xylella

• Sequence analysis of a 1296-nucleotide plasmid from Xylella fastidiosa [22].

References