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MeSH Review

Blood Stains

 
 
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High impact information on Blood Stains

  • Polymerase chain reaction amplification of DNA from aged blood stains: quantitative evaluation of the "suitability for purpose" of four filter papers as archival media [1].
  • When applied to blood stains and stored for various periods at room temperature, the stains up to 8 months old could still be phenotyped for EAP and those up to 4 weeks old for ESD [2].
  • Methods are described for phenotyping alpha 2HS-glycoprotein (A2HS) and group specific component (GC) in plasma and blood-stains [3].
  • In the blood stains, the proportion of cytosine was greater than thymine in both individuals [4].
  • The appropriate antiserum for detecting Rh C, c, D, E or e was coated on the inner surface of microplate wells, and the sample antigens from blood stains, solubilized with n-octyl-beta-D-glucopyranoside, were then placed in the wells [5].
 

Biological context of Blood Stains

 

Anatomical context of Blood Stains

  • We made an APC conjugate of monoclonal antibody 4F2, which reacts with an antigen abundant on the surfaces of activated human T-lymphocytes; APC-4F2 was used to stain blood mononuclear cells that had been cultured with and without phytohemagglutinin (PHA) [10].
 

Associations of Blood Stains with chemical compounds

  • A novel assay for typing Rh antigens in blood-stains using a lectin specific to the bisecting N-acetyl-D-glucosamine side chain of glycoprotein [5].
  • This paper describes a comparison of the performance of two standardized Romanowsky blood stains, namely those of Marshall et al. and Wittekind et al., both containing azure B and eosin alone [11].
  • Since the binding activity of actin to Gc protein is lost after treatment with a high concentration of guanidine HCl, the extracts of blood stains were treated with 4 M guanidine HCl to dissociate Gc protein and actin and then dialyzed to remove guanidine HCl [12].
  • Mineralized 100 microm cross sections were subjected to one of three treatments: unstained, stained with Villanueva's blood stain, and stained with acridine orange [13].
  • When used as a blood stain 0.4 ml borax methylene blue (1% methylene blue in 1% borax), 4 ml acetone, 2 ml borax-acid phosphate buffer to bring the solution to pH 6.5, and distilled water to make 40 ml, with 0.2 ml 1% eosin added just before using, an excellent Nocht-Giemsa type stain is achieved after 30 minutes staining [14].
 

Gene context of Blood Stains

  • The sensitivity of desialyzed AHSG phenotyping approaches that of GC and this technique is worthy of inclusion in blood stain screening protocols of forensic laboratories in regions where the population has a limited range of rare AHSG alleles [15].
  • The effect of temperature on Y-chromosome detection in blood stains [16].
  • DYS19 and amelogenin in artificial blood stains with defined amounts of male and female cells [17].
  • It was demonstrated that C6 phenotyping from blood stains aged over a period of 1 year, could be performed correctly [18].
  • OBJECTIVE: To assess the usefulness of heparin, alteplase, and streptokinase in removing blood stains [19].
 

Analytical, diagnostic and therapeutic context of Blood Stains

  • A sequence of tests of minute human blood stains for human origin identification and ABO blood grouping [20].
  • The present work deals with the factors affecting ABO grouping of dry blood stains in Riyad, including exposure to extremes of temperature, from refrigeration at -4 degrees C up to heating at 150 degrees C, effect of time till 6 months, occurrence of the stains on different fabrics, and effect of putrefaction [21].
  • Identification in blood stains through DNA typing with C4 and HLA-DR probes [22].

References

  1. Polymerase chain reaction amplification of DNA from aged blood stains: quantitative evaluation of the "suitability for purpose" of four filter papers as archival media. Kline, M.C., Duewer, D.L., Redman, J.W., Butler, J.M., Boyer, D.A. Anal. Chem. (2002) [Pubmed]
  2. Phenotyping of erythrocyte acid phosphatase and esterase D by high field strength isoelectric focusing on cellulose acetate membrane. Kane, M., Yamamoto, Y., Yamada, M., Fukunaga, T., Tatsuno, Y. Electrophoresis (1990) [Pubmed]
  3. The use of alpha 2HS-glycoprotein and group specific component in typing forensic blood samples for discriminative and investigative purposes. Westwood, S.A. Electrophoresis (1988) [Pubmed]
  4. A family exhibiting heteroplasmy in the human mitochondrial DNA control region reveals both somatic mosaicism and pronounced segregation of mitotypes. Wilson, M.R., Polanskey, D., Replogle, J., DiZinno, J.A., Budowle, B. Hum. Genet. (1997) [Pubmed]
  5. A novel assay for typing Rh antigens in blood-stains using a lectin specific to the bisecting N-acetyl-D-glucosamine side chain of glycoprotein. Matsubara, K., Tanabe, K., Akane, A., Nakamura, H., Takahashi, S., Kimura, K. J. Immunol. Methods (1994) [Pubmed]
  6. Errors in ABO typing of blood stains using PCR. Ringel, P.F., Weiler, G., Bein, G. Int. J. Legal Med. (2000) [Pubmed]
  7. Human identification and sex determination of dental pulp, bone marrow and blood stains with a recombinant DNA probe. Yokoi, T., Aoki, Y., Sagisaka, K. Z. Rechtsmed. (1989) [Pubmed]
  8. Human orosomucoid (ORM1) subtyping: further population genetic data and reports on the feasibility to type aged blood samples and stains. Dülmer, M., Reker, G., Nguyen, T.T., Henke, L., Henke, J. J. Forensic Sci. (1998) [Pubmed]
  9. Population study of HUMTH01, HUMVWA31/A, HUMF13A1, and HUMFES/FPS systems in Azores. Corte-Real, F., Souto, L., Anjos, M.J., Carvalho, M., Vieira, D.N., Carracedo, A., Vide, M.C. J. Forensic Sci. (1999) [Pubmed]
  10. Immunofluorescence measurement in a flow cytometer using low-power helium-neon laser excitation. Shapiro, H.M., Glazer, A.N., Christenson, L., Williams, J.M., Strom, T.B. Cytometry. (1983) [Pubmed]
  11. Microspectrophotometric studies of Romanowsky stained blood cells. II. Comparison of the performance of two standardized stains. Marshall, P.N., Galbraith, W., Navarro, E.F., Bacus, J.W. Journal of microscopy. (1981) [Pubmed]
  12. The typing of group-specific component (Gc protein) in human blood stains. Kimura, H., Shinomiya, K., Yoshida, K., Shinomiya, T. Forensic Sci. Int. (1983) [Pubmed]
  13. Determination of age from cemental incremental lines for forensic dentistry. Sousa, E.M., Stott, G.G., Alves, J.B. Biotechnic & histochemistry : official publication of the Biological Stain Commission. (1999) [Pubmed]
  14. Borax methylene blue: a spectroscopic and staining study. Donaldson, P.T., Russo, A., Reynolds, C., Lillie, R.D. Stain technology. (1978) [Pubmed]
  15. Routine phenotyping of native and desialyzed alpha 2HS-glycoprotein from blood stains. Thomas, A.S., Aaskov, J.G. Forensic Sci. Int. (1990) [Pubmed]
  16. The effect of temperature on Y-chromosome detection in blood stains. Thomsen, J.L. Forensic Sci. Int. (1980) [Pubmed]
  17. DYS19 and amelogenin in artificial blood stains with defined amounts of male and female cells. Zehner, R., Bohrer, U. Int. J. Legal Med. (1998) [Pubmed]
  18. Forensic application of blood stains and polymorphism of the sixth component of human complement (C6). Kishi, K., Sawazaki, K., Yasuda, T. Forensic Sci. Int. (1988) [Pubmed]
  19. Streptokinase versus alteplase and other treatments for acute and delayed thrombolysis of blood stains in clothing. Pager, C.K. BMJ (2000) [Pubmed]
  20. A sequence of tests of minute human blood stains for human origin identification and ABO blood grouping. Tokiwa, K. Z. Rechtsmed. (1986) [Pubmed]
  21. Study on the factors affecting ABO grouping of blood stains. el-Habashi, A.A., Jado, A., Farag, A.M., el-Assam, O. Journal of the Egyptian Society of Parasitology. (1991) [Pubmed]
  22. Identification in blood stains through DNA typing with C4 and HLA-DR probes. Olaisen, B., Mevåg, B., Jonassen, R., Paulsen, G., Thorsby, E., Teisberg, P. Z. Rechtsmed. (1987) [Pubmed]
 
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