Irradiation of fish embryos prior to blastomere transfer boosts the colonisation of their gonads by donor-derived gametes

Mol Reprod Dev. 1999 Aug;53(4):394-7. doi: 10.1002/(SICI)1098-2795(199908)53:4<394::AID-MRD4>3.0.CO;2-X.

Abstract

Blastomere transplantation into fish blastula embryos results in somatic chimeras, which generally provide null or a small proportion of gametes derived from the donor. This may partly explain why none of the ES-like cell lines established from fish embryos has contributed to the germline of chimeras when transplanted at the blastula stage. Here, we report that a moderate gamma-irradiation of recipient embryos, followed by transplantation of dispersed blastomeres, considerably enhances the proportion of donor-derived gametes (53% versus 5% in average). In fish, the resulting protocol should maximise the pluripotency level measured in vivo for embryonic cell lines and for cultured germ cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastomeres / transplantation*
  • Cell Transplantation / methods
  • Female
  • Gamma Rays
  • Gonads / embryology*
  • Gonads / radiation effects
  • Male
  • Oryzias / embryology*
  • Oryzias / genetics
  • Pigmentation
  • Radiation Chimera
  • Stem Cell Transplantation