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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Resonance energy transfer study using a rhenium metal-ligand lipid conjugate as the donor in a model membrane.

We measured steady state and time-resolved resonance energy transfer between donors and acceptors in model membranes. The donor was a long lifetime rhenium-lipid complex, which displayed a mean lifetime of 1 microsecond and lifetime components as long as 3 microseconds in the labeled DOPC membranes. The transfer efficiencies were found to be substantially larger than those predicted without consideration of lateral diffusion. The larger transfer efficiencies are consistent with a mutual lateral diffusion coefficient in the membrane near 2 x 10(-8) cm2/s. These results demonstrate that lateral diffusion in membranes can be detected with microsecond lipid probes.[1]

References

  1. Resonance energy transfer study using a rhenium metal-ligand lipid conjugate as the donor in a model membrane. Li, L., Gryczynski, I., Lakowicz, J.R. Chem. Phys. Lipids (1999) [Pubmed]
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