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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Hartman, C.P. et al.

Azo reduction of trypan blue to a known carcinogen by a cell-free extract of a human intestinal anaerobe.

The azo reductase activity of a cell-free extract of Fusobacterium sp. 2 is characterized using trypan blue as a substrate. Either chemical reduction of this dye with sodium hydrosulfite or reduction by the cell-free extract produces a mutagenic product, o-tolidine. The o-tolidine is mutagenic in the Ames Salmonella/mammalian-microsome mutagenicity test when activated by a rat liver S9 preparation.[1]

References

Azo reduction of trypan blue to a known carcinogen by a cell-free extract of a human intestinal anaerobe. Hartman, C.P., Fulk, G.E., Andrews, A.W. Mutat. Res. (1978) [Pubmed]

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