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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Temperature-sensitive suppressor mutations of the Escherichia coli DNA gyrase B protein.

Escherichia coli strain LE316 contains a mutation in gyrB that results in the substitution of Val164 to Gly and confers both chlorobiocin resistance and temperature sensitivity. Selection for suppressors of the ts phenotype yielded second-site mutations in GyrB at His38 and Thr157. The properties of proteins bearing these mutations have been characterized, and a mechanism of suppression is proposed based upon structural considerations.[1]

References

  1. Temperature-sensitive suppressor mutations of the Escherichia coli DNA gyrase B protein. Blance, S.J., Williams, N.L., Preston, Z.A., Bishara, J., Smyth, M.S., Maxwell, A. Protein Sci. (2000) [Pubmed]
 
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