Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Hoffmann-Sommergruber, K. et al.

Hoffmann-Sommergruber, Ferris, Pec, Radauer, O'Riordain, Laimer Da Camara Machado, Scheiner, Breiteneder,

Characterization of api g 1.0201, a new member of the Api g 1 family of celery allergens.

BACKGROUND: The association of pollinosis with allergy to plant foods occurs in up to 70% of tree pollen-allergic patients. In recent years, some of the relevant cross-reacting proteins have been characterized at the molecular and immunological level. Api g 1 has been identified as the celery homologue of the major birch pollen allergen, Bet v 1. Although a number of Bet v 1 isoforms have been characterized from birch pollen, little is known about isoforms of food allergens and their allergenic features. METHODS: Api g 1.0201, an isoform of Api g 1, was isolated from a cDNA library, cloned and sequenced. The cDNA was expressed in Escherichia coli and the purified recombinant protein was tested in immunoblots. RESULTS: Api g 1.0201 displays 72% sequence similarity to the previously identified Api g 1.0101 and consists of 159 amino acid residues. The sequence of Api g 1.0201 has five additional amino acid residues at the carboxy-terminus as compared to Api g 1.0101. Purified recombinant Api g 1.0201 is recognized by IgE from the sera of celery-allergic patients, as well as by the murine monoclonal anti-Bet v 1 antibody. In general, this isoform displays a weaker IgE-binding capacity than Api g 1.0101, as concluded from immunoblotting experiments. Results from inhibition assays revealed that IgE-binding to Api g 1.0201 is only slightly reduced by preincubation with either purified recombinant Api g 1.0101 or purified recombinant Bet v 1a. Total inhibition was only achieved when using purified natural Bet v 1. CONCLUSIONS: At present, little is known about the IgE-binding capacity of isoforms of Bet v 1 homologues of food allergens. Identification and characterization of such isoforms may help to contribute to a better understanding of food allergy and the observed cross-reactivity to pollen allergy.[1]

References

Characterization of api g 1.0201, a new member of the Api g 1 family of celery allergens. Hoffmann-Sommergruber, K., Ferris, R., Pec, M., Radauer, C., O'Riordain, G., Laimer Da Camara Machado, M., Scheiner, O., Breiteneder, H. Int. Arch. Allergy Immunol. (2000) [Pubmed]

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