Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Pampfer, S. et al.

Pampfer, Cordi, Vanderheyden, Van Der Smissen, Courtoy, Van Cauwenberge, Alexandre, Donnay, De Hertogh,

Expression and role of Bcl-2 in rat blastocysts exposed to high D-glucose.

Bcl-2 mRNA expression was detected in rat blastocysts by in situ hybridization. The distribution of mRNA expression was rather heterogenous, with approximately 2% of high-expressing cells. In vitro exposure to 28 mmol/l D-glucose for 24 h resulted in a significant increase in the proportion of these cells compared with control embryos in either 6 mmol/l D-glucose or 28 mmol/l D+L-glucose. Heterogeneity in the expression of Bcl-2 was also observed at the protein level by immunocytochemistry. Exposure to 28 mmol/l D-glucose significantly increased the incidence of chromatin degradation (karyolysis) and nuclear fragmentation (karyorhexis), two nuclear markers of apoptosis in rat blastocysts. When two different antisense oligodeoxynucleotides designed to block Bcl-2 expression were added to 28 mmol/1 D-glucose, the incidence of karyolysis (but not karyorhexis) was increased compared with embryos in 28 mmol/l D-glucose alone. These data suggest that Bcl-2 is involved in the protective response against the induction of karyolysis in blastocysts on their exposure to high concentrations of D-glucose in vitro, whereas karyorhexis appears to result from the activation of an intracellular pathway that is independent of Bcl-2.[1]

References

Expression and role of Bcl-2 in rat blastocysts exposed to high D-glucose. Pampfer, S., Cordi, S., Vanderheyden, I., Van Der Smissen, P., Courtoy, P.J., Van Cauwenberge, A., Alexandre, H., Donnay, I., De Hertogh, R. Diabetes (2001) [Pubmed]

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