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An, X. et al.

The effects of ectopic white and transformer expression on Drosophila courtship behavior.

The sex determining genes of Drosophila males and females function to establish the potential for sex-specific behaviors. Previous studies suggest that ectopic GAL4-directed misexpression of the female-specific isoform of the sex-determining gene transformer (tra) in specific sub-domains of an otherwise male brain can lead to bisexual courtship behavior, thus identifying brain domains that may mediate sex-specific behavior. However, expression of mini-white, the marker gene used in both P[GAL4] and P[UAS(G)] constructs, also induces males to court other males, questioning whether GAL4-mediated tra expression alone can induce bisexual behavior. Here we demonstrate the consequences of inducing mutations in the mini-white genes within P[GAL4] and P[UAS(G)] constructs to generate flies in which a white mutant phenotype is revealed. In these mini-white mutant strains, P[GAL4]-mediated transformer expression alone is both sufficient and necessary to generate bisexual behavior. In addition, using RT-PCR, we reveal the presence of female transcripts of doublesex and fruitless in the brains of otherwise male (XY) flies exhibiting P[GAL4]-directed tra-expression, demonstrating that P[GAL4]-directed tra is functional at the molecular level. We conclude that P[GAL4]-directed misexpression of tra is responsible for the bisexual behavior previously described and that this is mediated via sex-specific splicing of dsx and fru. Our results support the validity of such strategies for identifying regions of the fly brain that underlie sex-specific behaviors.[1]

References

The effects of ectopic white and transformer expression on Drosophila courtship behavior. An, X., Armstrong, J.D., Kaiser, K., O'Dell, K.M. J. Neurogenet. (2000) [Pubmed]

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