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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Time-resolved sperm responses to an egg peptide measured by stopped-flow fluorometry.

Speract, a decapeptide from sea urchin egg jelly, induces various sperm responses. Stopped-flow fluorometry was used to examine the binding of labeled speract and the intracellular changes in pH (pH(i)) and Ca2+ ([Ca2+]i) it induces in sperm. We observed significant time delays for the increase in pH(i) and [Ca2+]i induced by 200 nM speract (69 and 190 ms, respectively). Also, we found that the receptor undergoes a pH(i)-dependent affinity change at around 129 ms. These time delays probably reflect biochemical processes underlying each sperm response to speract that circumscribe the time sequence of the signaling events.[1]

References

  1. Time-resolved sperm responses to an egg peptide measured by stopped-flow fluorometry. Nishigaki, T., Zamudio, F.Z., Possani, L.D., Darszon, A. Biochem. Biophys. Res. Commun. (2001) [Pubmed]
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