Novel motility mutants of synechocystis strain PCC 6803 generated by in vitro transposon mutagenesis

J Bacteriol. 2001 Oct;183(20):6140-3. doi: 10.1128/JB.183.20.6140-6143.2001.

Abstract

We screened for transposon-generated mutants of Synechocystis sp. strain PCC 6803 that exhibited aberrant phototactic movement. Of the 300 mutants generated, about 50 have been partially characterized; several contained transposons in genes encoding chemotaxis-related proteins, while others mapped to novel genes. These novel genes and their possible roles in motility are discussed.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • ATP-Binding Cassette Transporters / genetics
  • Amino Acid Sequence
  • Cyanobacteria / physiology*
  • DNA Transposable Elements
  • Escherichia coli Proteins*
  • Genes, Bacterial
  • HSP70 Heat-Shock Proteins / genetics
  • Molecular Chaperones / genetics
  • Molecular Sequence Data
  • Movement / physiology*
  • Mutagenesis, Insertional
  • Mutation*
  • Sequence Homology, Amino Acid

Substances

  • ATP-Binding Cassette Transporters
  • DNA Transposable Elements
  • Escherichia coli Proteins
  • HSP70 Heat-Shock Proteins
  • Molecular Chaperones
  • dnaK protein, E coli