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Jen, J.F. et al.

Jen, Chang, Yang,

On-line microdialysis-high-performance liquid chromatographic determination of aniline and 2-chloroaniline in polymer industrial wastewater.

Determination of aniline and 2-chloroaniline in polymer industrial wastewater was examined using high-performance liquid chromatography with on-line microdialysis. After dilution, aniline and 2-chloroaniline in the sample were diffused through a cellular dialysis membrane into the perfusion stream under controlled conditions. Conditions for obtaining optimum dialysis efficiency such as flow-rate and polarity modifier in the perfusion stream, pH and added salt in the sample solution, as well as chromatographic conditions were investigated. The results indicate that the dialysis achieved at a sample matrix pH value of 9.5 with 0.1 M NaCl addition, and the perfusate at 10-microl/min flow-rate offered optimum dialysis efficiency. The aniline and 2-chloroaniline were well separated in an acceptable time on a reversed-phase C18 column eluted with 40% aqueous methanol solution at pH 7.0 and 1.0 ml/min flow-rate. The proposed method provided a very simple procedure to determine aniline and 2-chloroaniline in wastewater. Application was illustrated by the analysis of aniline and 2-chloroaniline in wastewater released from a polymer factory.[1]

References

On-line microdialysis-high-performance liquid chromatographic determination of aniline and 2-chloroaniline in polymer industrial wastewater. Jen, J.F., Chang, C.T., Yang, T.C. Journal of chromatography. A. (2001) [Pubmed]

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