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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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An in vitro screening method for DNA cytosine-C5-methylase inhibitor.

A specific inhibitor of DNA cytosine C5-methylases would be useful for studying genomic imprinting, X-chromosome inactivation, carcinogenesis, and regulation of tissue-specific gene expression, for these physiological phenomena appears to be regulated through DNA methylation in promoter sequences. This paper reports a novel convenient in vitro assay method for screening DNA cytosine C5-methylase inhibitor. Our method uses a commercially available Hae III methylase (cytosine C5 methylase), its corresponding Hae III endonuclease, and lambda DNA as their substrate.[1]

References

  1. An in vitro screening method for DNA cytosine-C5-methylase inhibitor. Tamura, T., Kataoka, A., Shu, L.Y., Ashida, A., Tanaka, H., Inagaki, K. Natural product letters. (2002) [Pubmed]
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