Simultaneous estimation of intracellular free Mg2+ and pH by use of a new pH-dependent dissociation constant of MgATP

Jpn J Physiol. 2002 Jun;52(3):323-6. doi: 10.2170/jjphysiol.52.323.

Abstract

In the present technical note for 31P-NMR, we used a new pH-dependent dissociation constant of MgATP, and re-estimated changes in the intracellular free Mg2+ concentration and pH from the chemical shifts of beta- and gamma-ATP during Na+-removal in smooth muscle. We confirmed the role of Na+ - Mg2+ exchange.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Animals
  • Cecum / metabolism*
  • Guinea Pigs
  • Hydrogen / metabolism*
  • Hydrogen-Ion Concentration
  • Intracellular Membranes / metabolism*
  • Magnesium / metabolism*
  • Models, Biological*
  • Sodium / pharmacology

Substances

  • Hydrogen
  • Adenosine Triphosphate
  • Sodium
  • Magnesium