Two-dimensional gel electrophoresis; better than a poke in the ICAT?

Curr Opin Biotechnol. 2002 Aug;13(4):321-8. doi: 10.1016/s0958-1669(02)00333-6.

Abstract

To date, the most widely used technology for conducting proteomic studies has been two-dimensional gel electrophoresis (2DGE), but this approach does have drawbacks. Isotope-coded affinity tagging (ICAT) is starting to challenge 2DGE as a new proteomic tool for the analysis of proteins in complex biological specimens. An appraisal of these two methodologies reveals that neither ICAT nor 2DGE provide comprehensive coverage on a proteome-wide scale.

Publication types

  • Comparative Study
  • Review

MeSH terms

  • Chromatography, Affinity / methods*
  • Chromatography, Affinity / trends
  • Electrophoresis, Gel, Two-Dimensional / methods*
  • Electrophoresis, Gel, Two-Dimensional / trends
  • Humans
  • Isotope Labeling / methods*
  • Macromolecular Substances
  • Mitochondrial Proton-Translocating ATPases / analysis*
  • Mitochondrial Proton-Translocating ATPases / metabolism
  • Oxidoreductases / analysis*
  • Oxidoreductases / metabolism
  • Proteins / analysis*
  • Proteins / metabolism
  • Proteome / analysis*
  • Quality Control

Substances

  • Macromolecular Substances
  • Proteins
  • Proteome
  • Oxidoreductases
  • Mitochondrial Proton-Translocating ATPases