Abstract
To date, the most widely used technology for conducting proteomic studies has been two-dimensional gel electrophoresis (2DGE), but this approach does have drawbacks. Isotope-coded affinity tagging (ICAT) is starting to challenge 2DGE as a new proteomic tool for the analysis of proteins in complex biological specimens. An appraisal of these two methodologies reveals that neither ICAT nor 2DGE provide comprehensive coverage on a proteome-wide scale.
MeSH terms
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Chromatography, Affinity / methods*
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Chromatography, Affinity / trends
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Electrophoresis, Gel, Two-Dimensional / methods*
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Electrophoresis, Gel, Two-Dimensional / trends
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Humans
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Isotope Labeling / methods*
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Macromolecular Substances
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Mitochondrial Proton-Translocating ATPases / analysis*
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Mitochondrial Proton-Translocating ATPases / metabolism
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Oxidoreductases / analysis*
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Oxidoreductases / metabolism
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Proteins / analysis*
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Proteins / metabolism
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Proteome / analysis*
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Quality Control
Substances
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Macromolecular Substances
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Proteins
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Proteome
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Oxidoreductases
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Mitochondrial Proton-Translocating ATPases